Details of the 40 field methods and the reference laboratory method
| Characteristic . | Number . |
|---|---|
| Regions where the methods were performed | |
| Australia/New Zealand | 13 |
| Asia | 10 |
| North America | 8 |
| Europe | 8 |
| South America | 1 |
| South Africa | 1 |
| Reference for the RQ-PCR methods | |
| Gabert et al,22 Beillard et al23 (EAC) | 17 |
| Branford et al2,21 | 12 |
| Emig et al1 | 3 |
| Emig et al,1 Müller et al25 | 1 |
| Radich et al,3 Hughes et al8 | 1 |
| Press et al11 | 1 |
| Stock et al24 | 1 |
| In house | 5 |
| Instruments | |
| Applied Biosystems | 27 |
| LightCycler | 10 |
| Corbett Rotorgene | 4 |
| Control genes | |
| ABL | 18 |
| BCR | 15 |
| GUS | 4 |
| G6PDH | 3 |
| β2M | 1 |
| RNA extraction | |
| Trizol reagent | 25 |
| Qiagen column-based methods | 10 |
| MagnaPure LC mRNA isolation kit | 2 |
| PAXgene stabilization | 1 |
| RNA Stat-60 | 1 |
| TRI reagent solution | 1 |
| Versagene RNA purification | 1 |
| Reverse transcriptase | |
| Superscript | 24 |
| MMLV | 11 |
| Roche Transcriptor | 3 |
| AMV | 2 |
| Omniscript/Sensiscript | 1 |
| RT primer | |
| Random hexamers | 38 |
| Gene specific | 2 |
| Random hexamers/Oligo dT | 1 |
| Material used for standard curve | |
| Plasmid | 31 |
| RNA | 5 |
| cDNA | 3 |
| No standard curve | 2 |
| Characteristic . | Number . |
|---|---|
| Regions where the methods were performed | |
| Australia/New Zealand | 13 |
| Asia | 10 |
| North America | 8 |
| Europe | 8 |
| South America | 1 |
| South Africa | 1 |
| Reference for the RQ-PCR methods | |
| Gabert et al,22 Beillard et al23 (EAC) | 17 |
| Branford et al2,21 | 12 |
| Emig et al1 | 3 |
| Emig et al,1 Müller et al25 | 1 |
| Radich et al,3 Hughes et al8 | 1 |
| Press et al11 | 1 |
| Stock et al24 | 1 |
| In house | 5 |
| Instruments | |
| Applied Biosystems | 27 |
| LightCycler | 10 |
| Corbett Rotorgene | 4 |
| Control genes | |
| ABL | 18 |
| BCR | 15 |
| GUS | 4 |
| G6PDH | 3 |
| β2M | 1 |
| RNA extraction | |
| Trizol reagent | 25 |
| Qiagen column-based methods | 10 |
| MagnaPure LC mRNA isolation kit | 2 |
| PAXgene stabilization | 1 |
| RNA Stat-60 | 1 |
| TRI reagent solution | 1 |
| Versagene RNA purification | 1 |
| Reverse transcriptase | |
| Superscript | 24 |
| MMLV | 11 |
| Roche Transcriptor | 3 |
| AMV | 2 |
| Omniscript/Sensiscript | 1 |
| RT primer | |
| Random hexamers | 38 |
| Gene specific | 2 |
| Random hexamers/Oligo dT | 1 |
| Material used for standard curve | |
| Plasmid | 31 |
| RNA | 5 |
| cDNA | 3 |
| No standard curve | 2 |