JAM-A marks LTR-HSCs in BM
| Cell population . | Cell no. . | Engrafted mice . | Total . | Myeloid cells . | T cells . | B cells . |
|---|---|---|---|---|---|---|
| KSL | 300 | 5/6 | 16.7 ± 10.6 | 15.6 ± 17.2 | 32.1 ± 19.8 | 28.0 ± 17.0 |
| KSL JAM-A− | 100 | 0/4 | — | — | — | — |
| KSL JAM-A− | 300 | 0/6 | — | — | — | — |
| KSL JAM-A− | 1000 | 0/6 | — | — | — | — |
| KSL JAM-A+ | 100 | 7/9 | 32.4 ± 26.4 | 29.7 ± 25.7 | 38.7 ± 25.3 | 24.6 ± 29.2 |
| KSL JAM-A+ | 300 | 4/4 | 77.4 ± 29.6 | 82.3 ± 20.8 | 73.8 ± 31.5 | 83.4 ± 17.1 |
| JAM-A+ | 100 | 4/7 | 15.4 ± 24.4 | 13.8 ± 21.9 | 10.4 ± 13.6 | 14.5 ± 24.4 |
| Cell population . | Cell no. . | Engrafted mice . | Total . | Myeloid cells . | T cells . | B cells . |
|---|---|---|---|---|---|---|
| KSL | 300 | 5/6 | 16.7 ± 10.6 | 15.6 ± 17.2 | 32.1 ± 19.8 | 28.0 ± 17.0 |
| KSL JAM-A− | 100 | 0/4 | — | — | — | — |
| KSL JAM-A− | 300 | 0/6 | — | — | — | — |
| KSL JAM-A− | 1000 | 0/6 | — | — | — | — |
| KSL JAM-A+ | 100 | 7/9 | 32.4 ± 26.4 | 29.7 ± 25.7 | 38.7 ± 25.3 | 24.6 ± 29.2 |
| KSL JAM-A+ | 300 | 4/4 | 77.4 ± 29.6 | 82.3 ± 20.8 | 73.8 ± 31.5 | 83.4 ± 17.1 |
| JAM-A+ | 100 | 4/7 | 15.4 ± 24.4 | 13.8 ± 21.9 | 10.4 ± 13.6 | 14.5 ± 24.4 |
Results of competitive hematopoietic stem-cell transplantation assay. Percentages of donor-derived cells of recipient mice were analyzed after 20 weeks of transplantation. Cell number indicates the test cells (B6-Ly5.1) that were transplanted with 2 × 105 competitive adult BM cells (B6-F1).