Overlapping skewing between the T-cell repertoires of the MLC and the posttransplantation patient samples
| Vβ . | Patient no. . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 17 . | 18 . | |
| 1 | −/+ | +/+ | NO | NO | +/+ | −/− | −/− | NO | +/+ | NO | −/− | ND | −/+ | −/− | ND | ND | NO | +/+ |
| 2 | ND | −/− | +/+ | +/+ | +/+ | −/− | −/− | −/+ | −/− | NO | −/+ | +/+ | −/− | +/− | NO | +/+ | −/− | +/+ |
| 3 | ND | ND | +/+ | ND | NO | −/− | −/+ | −/+ | −/− | +/+ | −/− | +/+ | −/+ | −/− | +/+ | NO | −/− | +/+ |
| 4 | −/− | −/− | −/− | +/+ | +/+ | −/− | ND | −/− | −/− | +/+ | NO | +/− | −/− | NO | −/− | ND | NO | +/− |
| 5 | −/+ | +/+ | −/+ | +/+ | −/+ | ND | NO | +/+ | −/− | ND | +/+ | +/+ | +/+ | +/− | +/− | ND | −/+ | −/− |
| 6 | +/+ | ND | +/− | +/+ | −/− | −/− | +/− | NO | −/+ | +/+ | +/+ | NO | +/+ | +/+ | ND | ND | −/+ | +/+ |
| 7 | ND | −/− | −/+ | NO | −/− | +/+ | −/− | ND | −/+ | ND | +/+ | NO | −/− | +/+ | ND | −/− | −/+ | ND |
| 8 | NO | NO | +/+ | +/+ | −/− | −/+ | ND | −/+ | −/− | ND | +/+ | +/+ | −/+ | ND | −/− | −/− | +/+ | −/− |
| 9 | +/+ | −/− | +/+ | +/+ | −/− | −/− | −/− | −/+ | −/− | +/+ | +/+ | −/− | −/+ | −/− | +/+ | +/− | −/+ | +/+ |
| 11 | ND | +/+ | +/− | +/+ | −/+ | −/− | +/+ | ND | −/+ | +/+ | −/− | +/+ | +/+ | −/− | +/+ | ND | +/+ | ND |
| 12 | +/+ | ND | +/+ | ND | +/+ | +/− | ND | −/+ | ND | +/+ | ND | +/− | −/− | −/− | ND | ND | ND | ND |
| 13 | −/− | −/+ | −/+ | −/− | −/− | ND | +/+ | −/− | +/+ | −/− | −/− | −/− | −/+ | −/− | −/− | −/− | ND | −/− |
| 14 | −/− | +/+ | ND | ND | −/− | −/+ | −/− | −/− | +/+ | ND | ND | ND | +/+ | −/− | ND | NO | +/+ | +/+ |
| 15 | NO | +/+ | +/+ | +/+ | −/− | −/− | +/+ | NO | −/− | +/+ | −/+ | +/+ | −/− | +/+ | −/+ | ND | −/− | NO |
| 16 | +/− | −/− | +/− | −/− | −/− | −/− | NO | ND | −/− | +/+ | ND | +/+ | NO | NO | +/− | +/+ | −/− | +/+ |
| 17 | −/− | +/+ | +/+ | NO | −/− | −/− | +/− | +/+ | −/− | ND | +/+ | −/− | −/− | −/− | ND | +/+ | −/− | +/− |
| 18 | +/+ | +/+ | ND | NO | −/− | −/− | ND | ND | −/− | ND | +/− | ND | ND | NO | ND | ND | NO | ND |
| 19 | +/+ | +/− | +/+ | ND | −/− | −/− | +/+ | −/+ | −/− | ND | −/− | +/+ | +/+ | +/+ | +/+ | ND | +/+ | NO |
| 21 | +/+ | +/+ | ND | +/+ | +/+ | +/+ | ND | ND | +/+ | ND | −/− | ND | ND | ND | ND | −/− | −/− | ND |
| 22 | ND | ND | ND | ND | −/− | +/− | −/+ | ND | −/− | ND | ND | ND | ND | −/− | ND | ND | −/− | +/− |
| 23 | +/+ | −/+ | −/− | +/+ | +/− | −/+ | −/− | +/+ | NO | −/+ | NO | +/+ | −/− | +/+ | −/+ | −/− | +/+ | +/+ |
| % | 70 | 80 | 67 | 71 | 71 | 50 | 50 | 50 | 80 | 80 | 67 | 69 | 83 | 50 | 57 | 50 | 71 | 57 |
| Vβ . | Patient no. . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 17 . | 18 . | |
| 1 | −/+ | +/+ | NO | NO | +/+ | −/− | −/− | NO | +/+ | NO | −/− | ND | −/+ | −/− | ND | ND | NO | +/+ |
| 2 | ND | −/− | +/+ | +/+ | +/+ | −/− | −/− | −/+ | −/− | NO | −/+ | +/+ | −/− | +/− | NO | +/+ | −/− | +/+ |
| 3 | ND | ND | +/+ | ND | NO | −/− | −/+ | −/+ | −/− | +/+ | −/− | +/+ | −/+ | −/− | +/+ | NO | −/− | +/+ |
| 4 | −/− | −/− | −/− | +/+ | +/+ | −/− | ND | −/− | −/− | +/+ | NO | +/− | −/− | NO | −/− | ND | NO | +/− |
| 5 | −/+ | +/+ | −/+ | +/+ | −/+ | ND | NO | +/+ | −/− | ND | +/+ | +/+ | +/+ | +/− | +/− | ND | −/+ | −/− |
| 6 | +/+ | ND | +/− | +/+ | −/− | −/− | +/− | NO | −/+ | +/+ | +/+ | NO | +/+ | +/+ | ND | ND | −/+ | +/+ |
| 7 | ND | −/− | −/+ | NO | −/− | +/+ | −/− | ND | −/+ | ND | +/+ | NO | −/− | +/+ | ND | −/− | −/+ | ND |
| 8 | NO | NO | +/+ | +/+ | −/− | −/+ | ND | −/+ | −/− | ND | +/+ | +/+ | −/+ | ND | −/− | −/− | +/+ | −/− |
| 9 | +/+ | −/− | +/+ | +/+ | −/− | −/− | −/− | −/+ | −/− | +/+ | +/+ | −/− | −/+ | −/− | +/+ | +/− | −/+ | +/+ |
| 11 | ND | +/+ | +/− | +/+ | −/+ | −/− | +/+ | ND | −/+ | +/+ | −/− | +/+ | +/+ | −/− | +/+ | ND | +/+ | ND |
| 12 | +/+ | ND | +/+ | ND | +/+ | +/− | ND | −/+ | ND | +/+ | ND | +/− | −/− | −/− | ND | ND | ND | ND |
| 13 | −/− | −/+ | −/+ | −/− | −/− | ND | +/+ | −/− | +/+ | −/− | −/− | −/− | −/+ | −/− | −/− | −/− | ND | −/− |
| 14 | −/− | +/+ | ND | ND | −/− | −/+ | −/− | −/− | +/+ | ND | ND | ND | +/+ | −/− | ND | NO | +/+ | +/+ |
| 15 | NO | +/+ | +/+ | +/+ | −/− | −/− | +/+ | NO | −/− | +/+ | −/+ | +/+ | −/− | +/+ | −/+ | ND | −/− | NO |
| 16 | +/− | −/− | +/− | −/− | −/− | −/− | NO | ND | −/− | +/+ | ND | +/+ | NO | NO | +/− | +/+ | −/− | +/+ |
| 17 | −/− | +/+ | +/+ | NO | −/− | −/− | +/− | +/+ | −/− | ND | +/+ | −/− | −/− | −/− | ND | +/+ | −/− | +/− |
| 18 | +/+ | +/+ | ND | NO | −/− | −/− | ND | ND | −/− | ND | +/− | ND | ND | NO | ND | ND | NO | ND |
| 19 | +/+ | +/− | +/+ | ND | −/− | −/− | +/+ | −/+ | −/− | ND | −/− | +/+ | +/+ | +/+ | +/+ | ND | +/+ | NO |
| 21 | +/+ | +/+ | ND | +/+ | +/+ | +/+ | ND | ND | +/+ | ND | −/− | ND | ND | ND | ND | −/− | −/− | ND |
| 22 | ND | ND | ND | ND | −/− | +/− | −/+ | ND | −/− | ND | ND | ND | ND | −/− | ND | ND | −/− | +/− |
| 23 | +/+ | −/+ | −/− | +/+ | +/− | −/+ | −/− | +/+ | NO | −/+ | NO | +/+ | −/− | +/+ | −/+ | −/− | +/+ | +/+ |
| % | 70 | 80 | 67 | 71 | 71 | 50 | 50 | 50 | 80 | 80 | 67 | 69 | 83 | 50 | 57 | 50 | 71 | 57 |
% indicates number of overlapping skewed Vβ families in the MLC and posttransplantation samples divided by total skewed MLC; +, overlapping skewed spectratype; −, no skewing; NO, no overlapping skewing (MLC and post sample skewed in different peak); and ND, not determined.