Summary of reconstitution experiments
| . | mAFKL cells . | mFLKL cells . |
|---|---|---|
| Engraftment | 8/8 | 3/3 |
| Long-term reconstitution | 8/8 | 3/3 |
| Donor chimerism, mean ± SD | 7.2 ± 10.6 | 19.3 ± 13.5 |
| Granulocytes | 8/8 | 3/3 |
| T cells | 5/8 | 3/3 |
| B cells | 7/8 | 3/3 |
| NK cells | 5/7* | 2/2* |
| . | mAFKL cells . | mFLKL cells . |
|---|---|---|
| Engraftment | 8/8 | 3/3 |
| Long-term reconstitution | 8/8 | 3/3 |
| Donor chimerism, mean ± SD | 7.2 ± 10.6 | 19.3 ± 13.5 |
| Granulocytes | 8/8 | 3/3 |
| T cells | 5/8 | 3/3 |
| B cells | 7/8 | 3/3 |
| NK cells | 5/7* | 2/2* |
Engraftment (ie, the proportion of RAG1−/− C57BL/6 [Ly5.1] recipient mice presenting donor-derived cells in the blood 4 weeks after the transfer of E12.5 to E13.5 mAFKL and mFLKL cells) is shown in the first row. Long-term reconstitution was considered to be present when a minimum of 0.5% of donor-derived cells were present in the bone marrow of recipient mice 4 months after transplantation. The proportion of recipient mice presenting myeloid cells (granulocytes) and lymphoid cells of donor origin is indicated for each source of KL cells.
One mouse was not tested for the presence of NK cells.