Impact of the RAG1-S723C mutation on lymphocyte development
| Genotype . | Thymocyte no., ×106 . | CD4+CD8+ thymocytes, % . | Splenocyte no., ×106 . | CD4+ splenic T cells, ×106 . | CD8+ splenic T cells, ×106 . | Pro-B cells, % of IgM− . | Pro-B cells, ×105 . | Pre-B cells, % of IgM− . | Pre-B cells, ×105 . |
|---|---|---|---|---|---|---|---|---|---|
| RAG1+/+, n = 5 | 200 ± 44 | 82.7 ± 3.6 | 71.6 ± 27 | 7.5 ± 3.8 | 3.9 ± 1.6 | 8.0 ± 0.4 | 0.4 ± 0.1 | 56.8 ± 4.9 | 2.9 ± 1.0 |
| RAG1+/S723C, n = 5 | 175.6 ± 68.9 | 81.7 ± 5.1 | 77.5 ± 23 | 8.2 ± 4.0 | 4.4 ± 1.6 | 9.8 ± 2.9 | 0.5 ± 0.2 | 52.4 ± 15.1 | 3.1 ± 1.8 |
| RAG1S723C/S723C, n = 8 | 4.6 ± 2.4 | 0.41 ± 0.89 | 18 ± 12.5 | 0.2 ± 0.1 | 0.07 ± 0.05 | 44.8 ± 5.5 | 1.2 ± 0.7 (P = .03) | 4.4 ± 0.6 | 0.18 ± 0.1 |
| Genotype . | Thymocyte no., ×106 . | CD4+CD8+ thymocytes, % . | Splenocyte no., ×106 . | CD4+ splenic T cells, ×106 . | CD8+ splenic T cells, ×106 . | Pro-B cells, % of IgM− . | Pro-B cells, ×105 . | Pre-B cells, % of IgM− . | Pre-B cells, ×105 . |
|---|---|---|---|---|---|---|---|---|---|
| RAG1+/+, n = 5 | 200 ± 44 | 82.7 ± 3.6 | 71.6 ± 27 | 7.5 ± 3.8 | 3.9 ± 1.6 | 8.0 ± 0.4 | 0.4 ± 0.1 | 56.8 ± 4.9 | 2.9 ± 1.0 |
| RAG1+/S723C, n = 5 | 175.6 ± 68.9 | 81.7 ± 5.1 | 77.5 ± 23 | 8.2 ± 4.0 | 4.4 ± 1.6 | 9.8 ± 2.9 | 0.5 ± 0.2 | 52.4 ± 15.1 | 3.1 ± 1.8 |
| RAG1S723C/S723C, n = 8 | 4.6 ± 2.4 | 0.41 ± 0.89 | 18 ± 12.5 | 0.2 ± 0.1 | 0.07 ± 0.05 | 44.8 ± 5.5 | 1.2 ± 0.7 (P = .03) | 4.4 ± 0.6 | 0.18 ± 0.1 |