Engraftment of CD34+38+ and CD34+38− fractions of AML
| AML . | Strain and IS . | CD34+CD38+ . | CD34+CD38− . | ||||
|---|---|---|---|---|---|---|---|
| Dose/mouse × 106 . | Equivalent dose of MNCs × 106 . | Mice with AML/mice transplanted . | Dose/mouse × 103 . | Equivalent dose of MNCs × 106 . | Mice with AML/mice transplanted . | ||
| 3 | A | 1.8 | 5.5 | 3/4 | 1.2 | 31 | 0/1 |
| 4* | B | 8.5 | 2.7 | 3/3 | 50 | 68 | 0/1† |
| 4* | A | 5 | 1.6 | 1/1 | 3.8 | 5.1 | 0/1† |
| 6 | A | 1.2 | 7.5 | 2/2 | NA | NA | NA |
| 7* | A | 4 | 7.7 | 2/2 | 4 | 8.3 | 0/1 |
| 7* | A | 3.7 | 7.1 | 2/2 | 2.2 | 4.7 | 0/1 |
| 8 | B | 2.6 | 9.3 | 4/4 | 500 | 8.3 | 1/1 |
| 9 | C | 1.3 | 1.7 | 1/1 | 7.5 | 3 | 1/1‡ |
| 10 | A | 3.5 | 6.5 | 1/2 | 1.1 | 20 | 0/1 |
| AML . | Strain and IS . | CD34+CD38+ . | CD34+CD38− . | ||||
|---|---|---|---|---|---|---|---|
| Dose/mouse × 106 . | Equivalent dose of MNCs × 106 . | Mice with AML/mice transplanted . | Dose/mouse × 103 . | Equivalent dose of MNCs × 106 . | Mice with AML/mice transplanted . | ||
| 3 | A | 1.8 | 5.5 | 3/4 | 1.2 | 31 | 0/1 |
| 4* | B | 8.5 | 2.7 | 3/3 | 50 | 68 | 0/1† |
| 4* | A | 5 | 1.6 | 1/1 | 3.8 | 5.1 | 0/1† |
| 6 | A | 1.2 | 7.5 | 2/2 | NA | NA | NA |
| 7* | A | 4 | 7.7 | 2/2 | 4 | 8.3 | 0/1 |
| 7* | A | 3.7 | 7.1 | 2/2 | 2.2 | 4.7 | 0/1 |
| 8 | B | 2.6 | 9.3 | 4/4 | 500 | 8.3 | 1/1 |
| 9 | C | 1.3 | 1.7 | 1/1 | 7.5 | 3 | 1/1‡ |
| 10 | A | 3.5 | 6.5 | 1/2 | 1.1 | 20 | 0/1 |
IS indicates immunosuppressive treatment given to mice in addition to sublethal irradiation; A, NOD/SCID/IL2rγ−/− mice treated with IVIG; B, NOD/SCID/β2m−/− mice treated with anti-CD122 antibody; C, NOD/SCID/β2m−/− mice treated with IVIG; Equivalent dose of MNCs, the equivalent dose of mononuclear cells from which the cells transplanted were derived; NA, not applicable because mouse died before analysis.
More than one experiment was performed on a particular sample.
Normal multi-lineage engraftment observed from the CD34+ CD38− fraction of sample 4 in both experiments.
Multilineage engraftment observed from the CD34+ CD38− fraction of sample 9.