Binding affinities of synthetic peptides
| HLA . | Position . | Length, mer . | Sequence . | Score . | Fluorescence index . |
|---|---|---|---|---|---|
| A*0201 | Aur-A271-279 | 9 | KIADFGWSV | 3911 | 0.93 |
| A*0201 | Aur-A63-71 | 9 | KLVSSHKPV | 243 | 0.23 |
| A*0201 | Aur-A207-215 | 9 | YLILEYAPL | 147 | 1.47 |
| A*0201 | WT17-15 | 9 | DLNALLPAV | 12 | 0.06 |
| A*0201 | CMVpp65495-503 | 9 | NLVPMVATV | 160 | 1.71 |
| A*2402 | Aur-A207-215 | 9 | YLILEYAPL | 6 | 0.99 |
| A*2402 | WT17-15 | 9 | DLNALLPAV | 0.18 | 0.02 |
| A*2402 | WT1235-243Y | 9 | CYTWNQMNL | 200 | 4.5 |
| HLA . | Position . | Length, mer . | Sequence . | Score . | Fluorescence index . |
|---|---|---|---|---|---|
| A*0201 | Aur-A271-279 | 9 | KIADFGWSV | 3911 | 0.93 |
| A*0201 | Aur-A63-71 | 9 | KLVSSHKPV | 243 | 0.23 |
| A*0201 | Aur-A207-215 | 9 | YLILEYAPL | 147 | 1.47 |
| A*0201 | WT17-15 | 9 | DLNALLPAV | 12 | 0.06 |
| A*0201 | CMVpp65495-503 | 9 | NLVPMVATV | 160 | 1.71 |
| A*2402 | Aur-A207-215 | 9 | YLILEYAPL | 6 | 0.99 |
| A*2402 | WT17-15 | 9 | DLNALLPAV | 0.18 | 0.02 |
| A*2402 | WT1235-243Y | 9 | CYTWNQMNL | 200 | 4.5 |
The binding affinities of synthetic peptides for HLA molecules were predicted by computer algorithms available on the National Institutes of Health BIMAS website (http://www-bimas.cit.nih.gov/molbio/hla_bind). The binding affinities of synthetic peptides for HLA molecules were evaluated by MHC stabilization assay as detailed in “HLA peptide–binding assay.”