Serum anti–vaccinia virus antibodies and anti–vaccinia virus memory B cells in blood, spleen, and bone marrow
| Individual . | Age, y . | Antivaccinia virus IgG (serum dilution ratio)† . | % vaccinia virus–specific memory B cells . | ||
|---|---|---|---|---|---|
| In blood . | In spleen . | In bone marrow . | |||
| Nonvaccinated | |||||
| C1 | 28 | 0.22 | <0.001* | — | — |
| C2 | 27 | 0.07 | <0.001* | — | — |
| C3 | 24 | nd | <0.013* | — | — |
| C4 | 24 | 0.04 | <0.030* | — | — |
| C5 | 23 | 0.12 | <0.010* | — | — |
| C6 | 20 | 0.08 | <0.004* | — | — |
| C7 | 20 | 0.05 | <0.010* | — | — |
| C8 | 17 | nd | <0.018* | <0.002* | — |
| C9 | 7 | nd | nd | <0.005* | — |
| Median | — | 0.075 | — | — | — |
| Vaccinated | |||||
| V1 | 59 | 0.27 | 0.037 | — | — |
| V2 | 56 | 0.28 | 0.110 | — | — |
| V3 | 53 | 1 | 0.088 | — | — |
| V4 | 53 | 0.38 | 0.112 | — | — |
| V5 | 53 | 0.10 | 0.134 | — | — |
| V6 | 48 | 0.07 | 0.062 | — | — |
| V7 | 48 | 0.49 | 0.010 | — | — |
| V8 | 45 | nd | 0.117 | — | — |
| V9 | 35 | 0.73 | 0.155 | — | — |
| V10 | 75 | 1.16 | 0.047 | 0.456 | — |
| V11 | 74 | 0.53 | nd | 0.157 | — |
| V12 | 66 | ni | nd | 0.309 | — |
| V13 | 59 | 0.40 | <0.012* | 0.068 | — |
| V14 | 58 | 0.29 | nd | 0.301 | — |
| V15 | 57 | nd | nd | 0.616 | — |
| V16 | 53 | nd | nd | 0.230 | — |
| V17 | 45 | 0.21 | nd | 0.238 | — |
| V18 | 31 | ni | 0.031 | 0.088 | — |
| V19 | 53 | 0.68 | 0.022 | — | 0.038 |
| V20 | 47 | 0.10 | 0.067 | — | 0.018 |
| Median | — | 0.380 | 0.067 | 0.238 | — |
| Individual . | Age, y . | Antivaccinia virus IgG (serum dilution ratio)† . | % vaccinia virus–specific memory B cells . | ||
|---|---|---|---|---|---|
| In blood . | In spleen . | In bone marrow . | |||
| Nonvaccinated | |||||
| C1 | 28 | 0.22 | <0.001* | — | — |
| C2 | 27 | 0.07 | <0.001* | — | — |
| C3 | 24 | nd | <0.013* | — | — |
| C4 | 24 | 0.04 | <0.030* | — | — |
| C5 | 23 | 0.12 | <0.010* | — | — |
| C6 | 20 | 0.08 | <0.004* | — | — |
| C7 | 20 | 0.05 | <0.010* | — | — |
| C8 | 17 | nd | <0.018* | <0.002* | — |
| C9 | 7 | nd | nd | <0.005* | — |
| Median | — | 0.075 | — | — | — |
| Vaccinated | |||||
| V1 | 59 | 0.27 | 0.037 | — | — |
| V2 | 56 | 0.28 | 0.110 | — | — |
| V3 | 53 | 1 | 0.088 | — | — |
| V4 | 53 | 0.38 | 0.112 | — | — |
| V5 | 53 | 0.10 | 0.134 | — | — |
| V6 | 48 | 0.07 | 0.062 | — | — |
| V7 | 48 | 0.49 | 0.010 | — | — |
| V8 | 45 | nd | 0.117 | — | — |
| V9 | 35 | 0.73 | 0.155 | — | — |
| V10 | 75 | 1.16 | 0.047 | 0.456 | — |
| V11 | 74 | 0.53 | nd | 0.157 | — |
| V12 | 66 | ni | nd | 0.309 | — |
| V13 | 59 | 0.40 | <0.012* | 0.068 | — |
| V14 | 58 | 0.29 | nd | 0.301 | — |
| V15 | 57 | nd | nd | 0.616 | — |
| V16 | 53 | nd | nd | 0.230 | — |
| V17 | 45 | 0.21 | nd | 0.238 | — |
| V18 | 31 | ni | 0.031 | 0.088 | — |
| V19 | 53 | 0.68 | 0.022 | — | 0.038 |
| V20 | 47 | 0.10 | 0.067 | — | 0.018 |
| Median | — | 0.380 | 0.067 | 0.238 | — |
nd indicates not done; ni, not interpretable (patient receiving intravenous Ig at the time of sample collection); and —, not applicable.
Maximum estimate for individuals for whom memory B cells were undetectable (“Methods”).
Dilution factor with respect to the positive control, see definition in “Methods.”