Correlation of IGHV mutation status with FCRL, CD38, and ZAP-70 expression
| . | FCRL1 . | FCRL2 . | FCRL3 . | FCRL5 . | CD38 . | ZAP-70 (T/B) . | ZAP-70 (%) . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UM | ||||||||||||||
| Median (range) | 9.53 (1.01-30.7) | 1.79 (1.00-3.90) | 2.04 (1.02-12.1) | 1.43 (1.00-2.72) | 49.8 (0.68-99.4) | 2.79 (0.85-6.28) | 26.9 (0.58-94.7) | |||||||
| Mean ± SD | 10.0 ± 7.05 | 1.93 ± 0.89 | 2.89 ± 2.36 | 1.52 ± 0.40 | 48.5 ± 36.6 | 2.82 ± 1.11 | 29.5 ± 19.1 | |||||||
| MT | ||||||||||||||
| Median (range) | 26.0 (1.58-45.5) | 8.89 (1.05-19.4) | 8.15 (1.22-26.9) | 3.45 (1.05-8.35) | 1.63 (0.71-99.9) | 4.80 (1.40-13.0) | 10.1 (1.00-59.9) | |||||||
| Mean ± SD | 24.2 ± 9.86 | 9.07 ± 3.73 | 9.49 ± 6.03 | 3.57 ± 1.31 | 11.3 ± 26.0 | 5.24 ± 2.17 | 14.6 ± 13.1 | |||||||
| Cutoff value | ≥ 15 | < 15 | ≥ 4.2 | < 4.2 | ≥ 4.0 | < 4.0 | ≥ 2.3 | < 2.3 | < 30% | ≥ 30% | ≥ 4.0 | < 4.0 | < 20% | ≥ 20% |
| UM (n = 52) | 7 | 45 | 0 | 52 | 7 | 45 | 2 | 50 | 19 | 33 | 5 | 47 | 14 | 38 |
| MT (n = 55) | 45 | 10 | 49 | 6 | 46 | 9 | 49 | 6 | 49 | 6 | 39 | 16 | 42 | 13 |
| Sensitivity, % | 81.8 | 89.1 | 83.6 | 89.1 | 89.1 | 70.9 | 76.4 | |||||||
| Specificity, % | 86.5 | 100 | 86.5 | 96.2 | 63.5 | 90.4 | 73.1 | |||||||
| PPV, % | 86.5 | 100 | 86.8 | 96.1 | 72.1 | 88.6 | 75.0 | |||||||
| NPV, % | 81.8 | 89.7 | 83.3 | 89.3 | 84.6 | 74.6 | 74.5 | |||||||
| Concordance, % | 84.1 | 94.4 | 85.0 | 92.5 | 76.6 | 80.4 | 74.8 | |||||||
| C-statistic | 0.861 | 0.964 | 0.877 | 0.946 | 0.803 | 0.864 | 0.760 | |||||||
| . | FCRL1 . | FCRL2 . | FCRL3 . | FCRL5 . | CD38 . | ZAP-70 (T/B) . | ZAP-70 (%) . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UM | ||||||||||||||
| Median (range) | 9.53 (1.01-30.7) | 1.79 (1.00-3.90) | 2.04 (1.02-12.1) | 1.43 (1.00-2.72) | 49.8 (0.68-99.4) | 2.79 (0.85-6.28) | 26.9 (0.58-94.7) | |||||||
| Mean ± SD | 10.0 ± 7.05 | 1.93 ± 0.89 | 2.89 ± 2.36 | 1.52 ± 0.40 | 48.5 ± 36.6 | 2.82 ± 1.11 | 29.5 ± 19.1 | |||||||
| MT | ||||||||||||||
| Median (range) | 26.0 (1.58-45.5) | 8.89 (1.05-19.4) | 8.15 (1.22-26.9) | 3.45 (1.05-8.35) | 1.63 (0.71-99.9) | 4.80 (1.40-13.0) | 10.1 (1.00-59.9) | |||||||
| Mean ± SD | 24.2 ± 9.86 | 9.07 ± 3.73 | 9.49 ± 6.03 | 3.57 ± 1.31 | 11.3 ± 26.0 | 5.24 ± 2.17 | 14.6 ± 13.1 | |||||||
| Cutoff value | ≥ 15 | < 15 | ≥ 4.2 | < 4.2 | ≥ 4.0 | < 4.0 | ≥ 2.3 | < 2.3 | < 30% | ≥ 30% | ≥ 4.0 | < 4.0 | < 20% | ≥ 20% |
| UM (n = 52) | 7 | 45 | 0 | 52 | 7 | 45 | 2 | 50 | 19 | 33 | 5 | 47 | 14 | 38 |
| MT (n = 55) | 45 | 10 | 49 | 6 | 46 | 9 | 49 | 6 | 49 | 6 | 39 | 16 | 42 | 13 |
| Sensitivity, % | 81.8 | 89.1 | 83.6 | 89.1 | 89.1 | 70.9 | 76.4 | |||||||
| Specificity, % | 86.5 | 100 | 86.5 | 96.2 | 63.5 | 90.4 | 73.1 | |||||||
| PPV, % | 86.5 | 100 | 86.8 | 96.1 | 72.1 | 88.6 | 75.0 | |||||||
| NPV, % | 81.8 | 89.7 | 83.3 | 89.3 | 84.6 | 74.6 | 74.5 | |||||||
| Concordance, % | 84.1 | 94.4 | 85.0 | 92.5 | 76.6 | 80.4 | 74.8 | |||||||
| C-statistic | 0.861 | 0.964 | 0.877 | 0.946 | 0.803 | 0.864 | 0.760 | |||||||
FCRL cutoff values were determined according to the ROC and CART methods. The indexes including sensitivity, specificity, PPV, NPV, concordance (concordance with IGHV mutation status), or C-statistic (predictive of model accuracy) were evaluated. A C-statistic of 1.0 indicates perfect predictive discrimination, while values greater than 0.9 indicate outstanding, 0.8 to 0.9 indicate excellent, and 0.7 to 0.8 indicate fair discrimination.44