Bone marrow trephine histology, erythropoietin levels and iron stores in patients enrolled in the MRC PT-1 studies
| . | MPL mutant . | MPL negative . | P (vs V617F+) . | P (vs V617F−) . | |
|---|---|---|---|---|---|
| V617F+ . | V617F− . | ||||
| Bone marrow trephine histology, no.* | 13 | 168 | 130 | ||
| Reticulin grade, mean plus or minus SD | 1.8 ± 1.0 | 1.8 ± 0.8 | 1.9 ± 0.9 | .9 | .6 |
| Megakaryocyte clusters and nuclear morphology, no.† | |||||
| Clusters (absent/loose/tight) | 1 / 6 / 6 | 22 / 99 / 47 | 12 / 67 / 51 | .3 | .8 |
| Pyknotic (absent/pres/predom) | 6 / 6 / 1 | 55 / 112 / 1 | 48 / 79 / 3 | .8 | .9 |
| Staghorn (absent/pres/predom) | 2 / 10 / 1 | 31 / 132 / 5 | 23 / 102 / 5 | .7 | .7 |
| Cloud-like (absent/pres/predom) | 5 / 8 / 0 | 46 / 118 / 4 | 44 / 85 / 1 | .4 | .8 |
| Dysplastic (absent/pres/predom) | 4 / 8 / 1 | 57 / 110 / 1 | 48 / 80 / 2 | .6 | .6 |
| Cellularity, no.‡ | |||||
| Overall (dec/normal/inc) | 3 / 5 / 5 | 1 / 36 / 131 | 2 / 43 / 85 | < .001 | .005 |
| Erythroid (dec/ normal/inc) | 3 / 9 / 1 | 5 / 90 / 73 | 6 / 77 / 47 | < .001 | .005 |
| Granulocytic (dec/normal/inc) | 3 / 6 / 4 | 3 / 74 / 91 | 2 / 76 / 52 | .009 | .07 |
| Megakaryocytic (+/++/+++) | 3 / 9 / 1 | 32 / 88 / 47 | 25 / 58 / 47 | .3 | .2 |
| Erythropoietin levels and iron stores | |||||
| Erythropoietin, U/L§ | |||||
| Mean plus or minus SD | 20.7 ± 16.2 | 9.8 ± 10.8 | 23.8 ± 28.9 | <.001 | .6 |
| Median | 17.4 | 6.9 | 14.9 | ||
| (10th-90th centile) | (6.3-33.8) | (3.2-17.3) | (7.9-42.7) | ||
| Mean cell volume, fL¶ | |||||
| Mean plus or minus SD | 89.6 ± 5.5 | 87.6 ± 6.4 | 89.4 ± 6.1 | .08 | .8 |
| Median | 89.5 | 88.3 | 89.4 | ||
| (10th-90th centile) | (83.1-96.9) | (79.8-94.2) | (83.5-95.2) | ||
| Mean cell volume, no. less than 80 fL (%)‡ | 2/32 (6.3%) | 41/391 (10.5%) | 4/312 (1.3%) | .4 | .2 |
| Ferritin, μg/L‖ | |||||
| Mean plus or minus SD | 94 ± 79 | 90 ± 91 | 90.7 ± 90 | .8 | .9 |
| Median | 54 | 58 | 91 | ||
| (10th-90th centile) | (19-213) | (23-192) | (29-201) | ||
| . | MPL mutant . | MPL negative . | P (vs V617F+) . | P (vs V617F−) . | |
|---|---|---|---|---|---|
| V617F+ . | V617F− . | ||||
| Bone marrow trephine histology, no.* | 13 | 168 | 130 | ||
| Reticulin grade, mean plus or minus SD | 1.8 ± 1.0 | 1.8 ± 0.8 | 1.9 ± 0.9 | .9 | .6 |
| Megakaryocyte clusters and nuclear morphology, no.† | |||||
| Clusters (absent/loose/tight) | 1 / 6 / 6 | 22 / 99 / 47 | 12 / 67 / 51 | .3 | .8 |
| Pyknotic (absent/pres/predom) | 6 / 6 / 1 | 55 / 112 / 1 | 48 / 79 / 3 | .8 | .9 |
| Staghorn (absent/pres/predom) | 2 / 10 / 1 | 31 / 132 / 5 | 23 / 102 / 5 | .7 | .7 |
| Cloud-like (absent/pres/predom) | 5 / 8 / 0 | 46 / 118 / 4 | 44 / 85 / 1 | .4 | .8 |
| Dysplastic (absent/pres/predom) | 4 / 8 / 1 | 57 / 110 / 1 | 48 / 80 / 2 | .6 | .6 |
| Cellularity, no.‡ | |||||
| Overall (dec/normal/inc) | 3 / 5 / 5 | 1 / 36 / 131 | 2 / 43 / 85 | < .001 | .005 |
| Erythroid (dec/ normal/inc) | 3 / 9 / 1 | 5 / 90 / 73 | 6 / 77 / 47 | < .001 | .005 |
| Granulocytic (dec/normal/inc) | 3 / 6 / 4 | 3 / 74 / 91 | 2 / 76 / 52 | .009 | .07 |
| Megakaryocytic (+/++/+++) | 3 / 9 / 1 | 32 / 88 / 47 | 25 / 58 / 47 | .3 | .2 |
| Erythropoietin levels and iron stores | |||||
| Erythropoietin, U/L§ | |||||
| Mean plus or minus SD | 20.7 ± 16.2 | 9.8 ± 10.8 | 23.8 ± 28.9 | <.001 | .6 |
| Median | 17.4 | 6.9 | 14.9 | ||
| (10th-90th centile) | (6.3-33.8) | (3.2-17.3) | (7.9-42.7) | ||
| Mean cell volume, fL¶ | |||||
| Mean plus or minus SD | 89.6 ± 5.5 | 87.6 ± 6.4 | 89.4 ± 6.1 | .08 | .8 |
| Median | 89.5 | 88.3 | 89.4 | ||
| (10th-90th centile) | (83.1-96.9) | (79.8-94.2) | (83.5-95.2) | ||
| Mean cell volume, no. less than 80 fL (%)‡ | 2/32 (6.3%) | 41/391 (10.5%) | 4/312 (1.3%) | .4 | .2 |
| Ferritin, μg/L‖ | |||||
| Mean plus or minus SD | 94 ± 79 | 90 ± 91 | 90.7 ± 90 | .8 | .9 |
| Median | 54 | 58 | 91 | ||
| (10th-90th centile) | (19-213) | (23-192) | (29-201) | ||
All bone marrow trephine biopsies were obtained at diagnosis.
pres indicates present; and predom, predominant.
Dec, decreased; inc, increased; +, ++, +++, increasing cellularity.
Based on 707 serum samples from trial entry. Normal range for serum erythropoietin, 5 to 25 U/L.
Normal range for mean cell volume, 80 to 100 fL.
Based on serum samples taken within 3 months of diagnosis (n = 182). Normal range for ferritin, 20 to 300 μg/L.