List of antibodies used
| Antigen . | Source (catalog no.) . | Host . | Dilution . | Pretreatment . | Incubation . | Detection . |
|---|---|---|---|---|---|---|
| GFP | Molecular Probes/Invitrogen (11122) | Rabbit | 1:40000 | — | 1 h at RT | anti–rabbit HRP, FITC-Tyr |
| GFP | Chemicon/Millipore, Billerica, MA (AB16901) | Chicken | 1:5000 | — | 1 h at RT | anti–chicken biotin, ABC, FITC-Tyr |
| GFP | Abcam, Cambridge, MA (Ab290) | Rabbit | 1:10000 | — | 1 h at RT | anti–rabbit HRP, FITC-Tyr |
| VWF | Novocastra, Newcastle Upon Tyne, United Kingdom (NCL-VWFp) | Rabbit | 1:500 | Microwave | Overnight at 4°C | anti–rabbit HRP, A594-Tyr |
| CD31 | PharMingen, San Diego, CA, (550274) | Rat | 1:200 | — | Overnight at 4°C | anti–rat A594, α-rat A647 |
| VE-cadherin | Santa Cruz Biotechnology, Santa Cruz, CA (Sc-6458) | Goat | 1:100 | — | Overnight at 4°C | anti–goat biotin, SA-A594 |
| Iba1 | WAKO, Richmond, VA (019-19741) | Rabbit | 1:1000 | — | 1 h at RT | anti–rabbit HRP, A594-Tyr |
| BrdU | Accurate, Westbury, NY (OBT0030) | Rat | 1:200 | Microwave | Overnight at 4°C | anti–rat HRP, A350-Tyr |
| Antigen . | Source (catalog no.) . | Host . | Dilution . | Pretreatment . | Incubation . | Detection . |
|---|---|---|---|---|---|---|
| GFP | Molecular Probes/Invitrogen (11122) | Rabbit | 1:40000 | — | 1 h at RT | anti–rabbit HRP, FITC-Tyr |
| GFP | Chemicon/Millipore, Billerica, MA (AB16901) | Chicken | 1:5000 | — | 1 h at RT | anti–chicken biotin, ABC, FITC-Tyr |
| GFP | Abcam, Cambridge, MA (Ab290) | Rabbit | 1:10000 | — | 1 h at RT | anti–rabbit HRP, FITC-Tyr |
| VWF | Novocastra, Newcastle Upon Tyne, United Kingdom (NCL-VWFp) | Rabbit | 1:500 | Microwave | Overnight at 4°C | anti–rabbit HRP, A594-Tyr |
| CD31 | PharMingen, San Diego, CA, (550274) | Rat | 1:200 | — | Overnight at 4°C | anti–rat A594, α-rat A647 |
| VE-cadherin | Santa Cruz Biotechnology, Santa Cruz, CA (Sc-6458) | Goat | 1:100 | — | Overnight at 4°C | anti–goat biotin, SA-A594 |
| Iba1 | WAKO, Richmond, VA (019-19741) | Rabbit | 1:1000 | — | 1 h at RT | anti–rabbit HRP, A594-Tyr |
| BrdU | Accurate, Westbury, NY (OBT0030) | Rat | 1:200 | Microwave | Overnight at 4°C | anti–rat HRP, A350-Tyr |
A350, A594, A647 indicate AlexaFluor dyes; ABC, avidin-biotin complex (Vector Laboratories, Burlingame, CA); TYR, tyramide conjugate; VWF, von Willebrand factor; and —, none.