Table 1 JAK2 V617F genotyping of blood... | American Society of Hematology

Table 1

JAK2 V617F genotyping of blood and bone marrow fractionated cell populations

Patient	Prognostic Lille score	Peripheral myeloid cells			Peripheral lymphoid cells			Bone marrow mononuclear cells	CD34⁺*	CD34⁺ CD38⁺*	CD34⁺ CD38⁻*
Patient	Prognostic Lille score	Gr	Pl	Mo	B	NK	T	Bone marrow mononuclear cells	CD34⁺*	CD34⁺ CD38⁺*	CD34⁺ CD38⁻*
IMF1	Low	++	++	++	++	−	−	ND	ND	ND	ND
IMF2	High	++	+++	++	++	+	−	ND	++	++	++
IMF3	High	++	++	++	++	++	+_low	ND	ND	ND	ND
IMF4	Int	++	++	++	+	−	−	ND	+	+	+
IMF5	Low	+	+	+	−	ND	ND	ND	ND	ND	ND
IMF6	Int	++	++	++	−	+	ND	ND	++	++	++
IMF7	High	++	++	++	+	++	+_low	ND	+	+	+
IMF8	Int	+	+	+	−	ND	−	ND	ND	ND	ND
IMF9	Int	+	+	+	−	−	−	ND	+	+	+
IMF10	Int	++	++	++	++	++	−	ND	++	++	++
PV1	NA	++	++	+	−	−	−	++	++	ND	ND
PV2	NA	++	+	+	−	−	−	ND	ND	ND	ND
PV3	NA	++	++	++	−	−	−	++	++	++	−
PV4	NA	++	++	++	−	−	−	+++	++	++	−
PV5	NA	++	++	++	−	−	−	+++	ND	ND	ND
PV6	NA	++	++	+	−	−	−	++	+	++	−
PV7	NA	+++	++	++	−	−	−	+++	ND	ND	ND
PV8	NA	+++	++	++	−	+	−	ND	ND	ND	ND
PV9	NA	+++	++	++	+	−	−	++	ND	ND	ND
PV10	NA	+++	++	++	−	−	−	+++	++	++	++
PV11	NA	++	ND	ND	ND	ND	ND	++	++	++	+
PV12	NA	+++	ND	ND	ND	ND	ND	+++	ND	ND	ND

Patient	Prognostic Lille score	Peripheral myeloid cells			Peripheral lymphoid cells			Bone marrow mononuclear cells	CD34⁺*	CD34⁺ CD38⁺*	CD34⁺ CD38⁻*
Patient	Prognostic Lille score	Gr	Pl	Mo	B	NK	T	Bone marrow mononuclear cells	CD34⁺*	CD34⁺ CD38⁺*	CD34⁺ CD38⁻*
IMF1	Low	++	++	++	++	−	−	ND	ND	ND	ND
IMF2	High	++	+++	++	++	+	−	ND	++	++	++
IMF3	High	++	++	++	++	++	+_low	ND	ND	ND	ND
IMF4	Int	++	++	++	+	−	−	ND	+	+	+
IMF5	Low	+	+	+	−	ND	ND	ND	ND	ND	ND
IMF6	Int	++	++	++	−	+	ND	ND	++	++	++
IMF7	High	++	++	++	+	++	+_low	ND	+	+	+
IMF8	Int	+	+	+	−	ND	−	ND	ND	ND	ND
IMF9	Int	+	+	+	−	−	−	ND	+	+	+
IMF10	Int	++	++	++	++	++	−	ND	++	++	++
PV1	NA	++	++	+	−	−	−	++	++	ND	ND
PV2	NA	++	+	+	−	−	−	ND	ND	ND	ND
PV3	NA	++	++	++	−	−	−	++	++	++	−
PV4	NA	++	++	++	−	−	−	+++	++	++	−
PV5	NA	++	++	++	−	−	−	+++	ND	ND	ND
PV6	NA	++	++	+	−	−	−	++	+	++	−
PV7	NA	+++	++	++	−	−	−	+++	ND	ND	ND
PV8	NA	+++	++	++	−	+	−	ND	ND	ND	ND
PV9	NA	+++	++	++	+	−	−	++	ND	ND	ND
PV10	NA	+++	++	++	−	−	−	+++	++	++	++
PV11	NA	++	ND	ND	ND	ND	ND	++	++	++	+
PV12	NA	+++	ND	ND	ND	ND	ND	+++	ND	ND	ND

Prognostic score of IMF patients (Lille score)²⁴ is indicated.

Gr indicates granulocytes; Pl, platelets; Mo, monocytes; +++, samples with > 50% of mutated allele; ++, samples with mutated allele ranging from 25% to 50%; +, samples with 5% to 25% of mutated allele; +_low, positive samples with mutated allele at the threshold of detection; −, absence of the JAK2 V617F mutation; ND, no data; NA, not applicable.

CD34⁺, CD34⁺CD38⁺, and CD34⁺CD38^– cells were separated from whole blood in IMF patients, and from bone marrow samples in PV patients.

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