Significant enhancement of d.a.MKK6-induced CD86 and CD80 expression by p100ΔN
| Experiment* . | Ctrl . | MKK6 . | MKK6 + p100ΔN . | P‡ . | |
|---|---|---|---|---|---|
| Ctrl vs MKK6 . | MKK6 vs MKK6 + p100ΔN . | ||||
| % CD86†; | .006 | .033 | |||
| 1 | 36 | 83 | 90 | ||
| 2 | 5 | 70 | 85 | ||
| 3 | 1 | 43 | 54 | ||
| 4 | 11 | 45 | 70 | ||
| % CD80† | .026 | .014 | |||
| 1 | 27 | 71 | 88 | ||
| 2 | 5 | 72 | 88 | ||
| 3 | 2 | 41 | 54 | ||
| 4 | 31 | 49 | 78 | ||
| Experiment* . | Ctrl . | MKK6 . | MKK6 + p100ΔN . | P‡ . | |
|---|---|---|---|---|---|
| Ctrl vs MKK6 . | MKK6 vs MKK6 + p100ΔN . | ||||
| % CD86†; | .006 | .033 | |||
| 1 | 36 | 83 | 90 | ||
| 2 | 5 | 70 | 85 | ||
| 3 | 1 | 43 | 54 | ||
| 4 | 11 | 45 | 70 | ||
| % CD80† | .026 | .014 | |||
| 1 | 27 | 71 | 88 | ||
| 2 | 5 | 72 | 88 | ||
| 3 | 2 | 41 | 54 | ||
| 4 | 31 | 49 | 78 | ||
CD34+ cells from 4 individual cord-blood donor samples (experiments 1 to 4).
Percentages of gated CD1a+GFP+NGFR+ LCs transduced with control vectors (ctrl), d.a.MKK6 + ctrl (MKK6), or d.a.MKK6 + p100ΔN positive for CD86 or CD80 expression.
The paired t test was performed for statistical analysis of CD86 or CD80 expression.