Frequency of GFP+ cells in circulation throughout development
| Embryonic stage . | Circulating blood cells expressing GFP, % . |
|---|---|
| E9.5 | 97 ± 2 |
| E10.5 | 95 ± 1 |
| E11.5 | 89 ± 8 |
| E12.5 | 77 ± 6 |
| E13.5 | 69 ± 5 |
| E14.5 | 51 ± 9 |
| E15.5 | 28 ± 10 |
| E16.5 | 5 ± 2 |
| E17.5 | 3 ± 0.8 |
| E18.5 | 2 ± 0.8 |
| P0 | 2 ± 0.6 |
| P8 | 0.6 ± 0.2 |
| P21 | 0.5 ± 0.2 |
| Adult* | 0.4 |
| Embryonic stage . | Circulating blood cells expressing GFP, % . |
|---|---|
| E9.5 | 97 ± 2 |
| E10.5 | 95 ± 1 |
| E11.5 | 89 ± 8 |
| E12.5 | 77 ± 6 |
| E13.5 | 69 ± 5 |
| E14.5 | 51 ± 9 |
| E15.5 | 28 ± 10 |
| E16.5 | 5 ± 2 |
| E17.5 | 3 ± 0.8 |
| E18.5 | 2 ± 0.8 |
| P0 | 2 ± 0.6 |
| P8 | 0.6 ± 0.2 |
| P21 | 0.5 ± 0.2 |
| Adult* | 0.4 |
Individual embryos were exsanguinated at different stages of development and peripheral blood was examined for the presence of GFP+ (primitive erythroid) cells by FACS. Percentages are expressed as mean ± SEM per embryo.
Two-year-old mouse.