Decreased expression of genes essential for HSC function in Cited2−/− Lin−c-Kit+ cells at 13.5 dpc
| Gene name . | Real-time PCR Lin−c-Kit+ cells . |
|---|---|
| Bmi-1 | -6.08 ± 0.81 |
| GATA-1 | ND |
| GATA2 | -4.42 ± 0.36 |
| GATA3 | ND |
| LEF-1 | -15.5 ± 0.67 |
| Mcl-1 | -4.13 ± 0.1 |
| Notch1 | -2.9 ± 0.25 |
| BMP4 | NC |
| Gene name . | Real-time PCR Lin−c-Kit+ cells . |
|---|---|
| Bmi-1 | -6.08 ± 0.81 |
| GATA-1 | ND |
| GATA2 | -4.42 ± 0.36 |
| GATA3 | ND |
| LEF-1 | -15.5 ± 0.67 |
| Mcl-1 | -4.13 ± 0.1 |
| Notch1 | -2.9 ± 0.25 |
| BMP4 | NC |
Real-time PCR was performed to analyze an array of genes essential for HSC function. HPRT was used as an internal control for real-time PCR, and the expression of each gene was normalized to HPRT and compared between Cited2−/− and wild-type littermate controls. The table shows mean (± SD) fold changes in Cited2−/− (n = 3) Lin−c-Kit+ cells at 13.5 dpc after comparison with wild-type littermate controls (n = 3).
ND indicates not detected; NC, no change.