Decreased expression of genes restricted to myeloid lineage in Cited2−/− fetal liver and Lin−c-Kit+ cells at 13.5 dpc
| Gene name . | Microarray . | Real-time PCR . | |
|---|---|---|---|
| Fetal liver . | Fetal liver . | Lin−c-Kit+ cells . | |
| Pre-pro proteinase3 | −2.99 | −2.78 ± 0.11 | −2.9 ± 0.35 |
| Myeloperoxidase | −2.53 | −2.14 ± 0.02 | −4.28 ± 0.16 |
| Neutrophil elastase | −3.276 | −3.275 ± 0.46 | −5.77 ± 0.47 |
| Cathepsin G | −2.738 | −2.015 ± 0.12 | −3.47 ± 0.07 |
| Eosinophil peroxidase | −12.931 | −6 ± 1.47 | −6.12 ± 0.22 |
| Wnt5a | −9.413 | −4.68 ± 1.39 | ND |
| Gene name . | Microarray . | Real-time PCR . | |
|---|---|---|---|
| Fetal liver . | Fetal liver . | Lin−c-Kit+ cells . | |
| Pre-pro proteinase3 | −2.99 | −2.78 ± 0.11 | −2.9 ± 0.35 |
| Myeloperoxidase | −2.53 | −2.14 ± 0.02 | −4.28 ± 0.16 |
| Neutrophil elastase | −3.276 | −3.275 ± 0.46 | −5.77 ± 0.47 |
| Cathepsin G | −2.738 | −2.015 ± 0.12 | −3.47 ± 0.07 |
| Eosinophil peroxidase | −12.931 | −6 ± 1.47 | −6.12 ± 0.22 |
| Wnt5a | −9.413 | −4.68 ± 1.39 | ND |
Microarray data collected from Cited2−/− fetal liver (n=3) and Cited2+/+ littermate controls (n=3) were compared, and real-time PCR was performed to verify the expression of differentially expressed genes. HPRT was used as an internal control for real-time PCR, and the expression of each gene was normalized to HPRT and compared between Cited2−/− and Cited2+/+ littermate controls. The table shows mean (± SD) fold changes in Cited2−/− (n = 3-5) fetal liver at 14.5 dpc and Lin−c-Kit+ cells at 13.5 dpc after comparison with wild-type littermate controls (n = 3-5).
ND indicates not detected.