CRU frequency in Cited2+/+ and Cited2−/− fetal liver LSK cells
| Cell dose injected . | No. of mice with donor-derived reconstitution . | No. of mice analyzed . | CRUfrequency . | 95% Cl . |
|---|---|---|---|---|
| +/+ | ||||
| 20 | 0 | 2 | — | — |
| 50 | 2 | 5 | — | — |
| 100 | 3 | 4 | — | — |
| 300 | 5 | 5 | — | — |
| 1/83 | 1/39-1/175 | |||
| −/− | ||||
| 20 | 0 | 5 | — | — |
| 50 | 0 | 4 | — | — |
| 100 | 0 | 5 | — | — |
| 300 | 1 | 5 | — | — |
| 1/2147 | 1/307-1/14 988* | |||
| Cell dose injected . | No. of mice with donor-derived reconstitution . | No. of mice analyzed . | CRUfrequency . | 95% Cl . |
|---|---|---|---|---|
| +/+ | ||||
| 20 | 0 | 2 | — | — |
| 50 | 2 | 5 | — | — |
| 100 | 3 | 4 | — | — |
| 300 | 5 | 5 | — | — |
| 1/83 | 1/39-1/175 | |||
| −/− | ||||
| 20 | 0 | 5 | — | — |
| 50 | 0 | 4 | — | — |
| 100 | 0 | 5 | — | — |
| 300 | 1 | 5 | — | — |
| 1/2147 | 1/307-1/14 988* | |||
Lethally irradiated B6.SJL/BoyJ recipient mice (5 recipient mice for each cell dose) received a transplant of sorted LSK cells (CD45.2+) from Cited2+/+ (n = 5) and Cited2−/− (n = 4) fetal liver at 14.5 dpc at different cell doses as indicated. Donor-derived reconstitution was assessed by monitoring the percentage for CD45.2+ lymphoid (B220+) and myeloid (Gr-1+ and Mac-1+) cells in the peripheral blood at 15 weeks after transplantation. Mice that had more than 1% test sample–derived (CD45.2+) cells in both lymphoid and myeloid subpopulations were considered to be repopulated by test donor cells. The CRU frequency in the test fetal liver LSK cells was calculated by L-Calc software.
CI indicates confidence interval; —, not applicable.
P = .002.