Real-time PCR primers
| Primer . | Forward . | Reverse . |
|---|---|---|
| Cited2 | 5′-cgcatcatcaccagcagcag-3′ | 5′-cgctcgtggcattcatgttg-3′ |
| PRTN3 | 5′-agattgtaggtgggcacgag-3′ | 5′-agcaccactgtcacaagctg-3′ |
| Neutrophil elastase | 5′-actctggctgccatgctact-3′ | 5′-ccggaaatttagaccgttca-3′ |
| MPO | 5′-caccctctttgttcgagagc-3′ | 5′-caacaccaagggcaggtagt-3′ |
| Cathepsin G | 5′-tctcctgctcctgttgacct-3′ | 5′-cctttctcgcatttggatgt-3′ |
| Eosinophil peroxidase | 5′-ccgacaacattgacatctgg-3′ | 5′-tgaaaactccccatttctgc-3′ |
| Wnt5a | 5′-caaataggcagccgagagac-3′ | 5′-tctagcgtccacgaactcct-3′ |
| LEF-1 | 5′-tcactgtcaggcgacacttc-3′ | 5′-tgaggcttcacgtgcattag-3′ |
| GATA1 | 5′-gatggaatccagacgaggaa-3′ | 5′-gccctgacagtaccacaggt-3′ |
| GATA2 | 5′-ccagcaaatccaagaagagc-3′ | 5′-agactggaggaagggtggat-3′ |
| GATA3 | 5′-ctggaggaggaacgctaatg-3′ | 5′-cagggatgacatgtgtctgg-3′ |
| Mcl-1 | 5′-agagcgctggagaccctg-3′ | 5′-ctatcttattagatatgccagacc-3′ |
| Notch1 | 5′-attgacgagtgtgaccctga-3′ | 5′-ggcactcattgatgttgggtc-3′ |
| Bmi-1 | 5′-gctctccagcattcgtcagtc-3′ | 5′-agcagcaatgactgtgatgca-3′ |
| BMP4 | 5′-tgatacctgagaccgggaag-3′ | 5′-ctgctcttcctcctcctcct-3′ |
| HPRT | 5′-gttggatacaggccagactttgttg-3′ | 5′-gagggtaggctggcctataggct-3′ |
| 18S | 5′-cgtctgccctatcaactttcg-3′ | 5′-ccttggatgtggtagccgtt-3′ |
| Primer . | Forward . | Reverse . |
|---|---|---|
| Cited2 | 5′-cgcatcatcaccagcagcag-3′ | 5′-cgctcgtggcattcatgttg-3′ |
| PRTN3 | 5′-agattgtaggtgggcacgag-3′ | 5′-agcaccactgtcacaagctg-3′ |
| Neutrophil elastase | 5′-actctggctgccatgctact-3′ | 5′-ccggaaatttagaccgttca-3′ |
| MPO | 5′-caccctctttgttcgagagc-3′ | 5′-caacaccaagggcaggtagt-3′ |
| Cathepsin G | 5′-tctcctgctcctgttgacct-3′ | 5′-cctttctcgcatttggatgt-3′ |
| Eosinophil peroxidase | 5′-ccgacaacattgacatctgg-3′ | 5′-tgaaaactccccatttctgc-3′ |
| Wnt5a | 5′-caaataggcagccgagagac-3′ | 5′-tctagcgtccacgaactcct-3′ |
| LEF-1 | 5′-tcactgtcaggcgacacttc-3′ | 5′-tgaggcttcacgtgcattag-3′ |
| GATA1 | 5′-gatggaatccagacgaggaa-3′ | 5′-gccctgacagtaccacaggt-3′ |
| GATA2 | 5′-ccagcaaatccaagaagagc-3′ | 5′-agactggaggaagggtggat-3′ |
| GATA3 | 5′-ctggaggaggaacgctaatg-3′ | 5′-cagggatgacatgtgtctgg-3′ |
| Mcl-1 | 5′-agagcgctggagaccctg-3′ | 5′-ctatcttattagatatgccagacc-3′ |
| Notch1 | 5′-attgacgagtgtgaccctga-3′ | 5′-ggcactcattgatgttgggtc-3′ |
| Bmi-1 | 5′-gctctccagcattcgtcagtc-3′ | 5′-agcagcaatgactgtgatgca-3′ |
| BMP4 | 5′-tgatacctgagaccgggaag-3′ | 5′-ctgctcttcctcctcctcct-3′ |
| HPRT | 5′-gttggatacaggccagactttgttg-3′ | 5′-gagggtaggctggcctataggct-3′ |
| 18S | 5′-cgtctgccctatcaactttcg-3′ | 5′-ccttggatgtggtagccgtt-3′ |