SNAP-23 and syntaxin-2 levels in permeabilized and nonpermeabilized platelets
| . | Nonpermeabilized . | Permeabilized* . |
|---|---|---|
| SNAP-23 | ||
| No addition | 1.5 ± 0.4‡ | 8.9 ± 4.3 |
| SFLLRN | 2.4 ± 0.4 | 9.3 ± 4.2 |
| P† | < .02 | ≥ .5 |
| Syntaxin-2 | ||
| No addition | 1.1 ± 0.1 | 6.1 ± 0.6 |
| SFLLRN | 2.4 ± 1.0 | 9.2 ± 4.0 |
| P† | < .02 | = .18 |
| . | Nonpermeabilized . | Permeabilized* . |
|---|---|---|
| SNAP-23 | ||
| No addition | 1.5 ± 0.4‡ | 8.9 ± 4.3 |
| SFLLRN | 2.4 ± 0.4 | 9.3 ± 4.2 |
| P† | < .02 | ≥ .5 |
| Syntaxin-2 | ||
| No addition | 1.1 ± 0.1 | 6.1 ± 0.6 |
| SFLLRN | 2.4 ± 1.0 | 9.2 ± 4.0 |
| P† | < .02 | = .18 |
Platelets were permeabilized by incubation with 3 μg/mL digitonin, stained with nonimmune IgG or IgG directed at the indicated tSNARE, and analyzed by flow cytometry.
P values indicate the statistical significance of the difference between platelets stimulated with SFLLRN versus untreated platelets as determined by the Student t test.
Values are reported as percent of control compared with signal observed in samples exposed to nonimmune antibodies.