Histogram statistics of FACS analysis of erythrocytes incubated with an FITC-labeled anti-CD47 antibody
. | Mean . | Geometrical mean . | SD . | CV . | Median . | Peak . | Peak channel . |
---|---|---|---|---|---|---|---|
Wt | |||||||
Mean | 75.4 | 68.8 | 42.8 | 56.6 | 70.4 | 653.0 | 68.8 |
SD | 2.8 | 1.7 | 6.2 | 6.0 | 1.7 | 9.9 | 3.3 |
tg6 | |||||||
Mean | 65.8 | 59.1 | 41.0 | 62.4 | 61.2 | 588.0 | 58.2 |
SD | 2.5 | 2.3 | 4.8 | 7.1 | 2.3 | 23.6 | 4.1 |
P | .001 | < .001 | .612 | 0.186 | < .001 | .001 | .002 |
. | Mean . | Geometrical mean . | SD . | CV . | Median . | Peak . | Peak channel . |
---|---|---|---|---|---|---|---|
Wt | |||||||
Mean | 75.4 | 68.8 | 42.8 | 56.6 | 70.4 | 653.0 | 68.8 |
SD | 2.8 | 1.7 | 6.2 | 6.0 | 1.7 | 9.9 | 3.3 |
tg6 | |||||||
Mean | 65.8 | 59.1 | 41.0 | 62.4 | 61.2 | 588.0 | 58.2 |
SD | 2.5 | 2.3 | 4.8 | 7.1 | 2.3 | 23.6 | 4.1 |
P | .001 | < .001 | .612 | 0.186 | < .001 | .001 | .002 |
For each individual histogram (n = 5–6; y-axis: number of events, x-axis: fluorescence intensity), eg, the average fluorescence intensity (Mean as column heading) and standard deviation (SD as column heading) has been calculated. In turn, these values were used to calculate the mean (Mean as row heading) and standard deviation (SD as row heading) of the means (column 1) or standard deviations (column 3), respectively, of all individual experiments.