Immunophenotype of iDCZol+ and mDCZol+
| . | iDCZol−* . | iDCZol+ . | mDCZol−† . | mDCZol+ . |
|---|---|---|---|---|
| HLA-DR | 680 ± 283 | 622 ± 249 | 1.287 ± 586 | 1.698 ± 910‡ |
| CD86 | 78 ± 25 | 142 ± 55 | 113 ± 30 | 121 ± 30 |
| CD80 | 235 ± 57 | 246 ± 55 | 263 ± 57 | 349 ± 85‡ |
| CD54 | 1.617 ± 341 | 1.685 ± 487 | 2.219 ± 780 | 2.168 ± 520 |
| CD83 | 82 ± 23 | 59 ± 15 | 139 ± 69 | 121 ± 46 |
| CD11a | 116 ± 58 | 113 ± 55 | 121 ± 48 | 619 ± 517 |
| CCR7 | 367 ± 35 | 368 ± 13 | 356 ± 36 | 317 ± 25 |
| CXCR4 | 132 ± 58 | 128 ± 57 | 136 ± 66 | 136 ± 72 |
| DC-LAMP | 58 ± 2 | 54 ± 5 | 78 ± 10 | 88 ± 2 |
| . | iDCZol−* . | iDCZol+ . | mDCZol−† . | mDCZol+ . |
|---|---|---|---|---|
| HLA-DR | 680 ± 283 | 622 ± 249 | 1.287 ± 586 | 1.698 ± 910‡ |
| CD86 | 78 ± 25 | 142 ± 55 | 113 ± 30 | 121 ± 30 |
| CD80 | 235 ± 57 | 246 ± 55 | 263 ± 57 | 349 ± 85‡ |
| CD54 | 1.617 ± 341 | 1.685 ± 487 | 2.219 ± 780 | 2.168 ± 520 |
| CD83 | 82 ± 23 | 59 ± 15 | 139 ± 69 | 121 ± 46 |
| CD11a | 116 ± 58 | 113 ± 55 | 121 ± 48 | 619 ± 517 |
| CCR7 | 367 ± 35 | 368 ± 13 | 356 ± 36 | 317 ± 25 |
| CXCR4 | 132 ± 58 | 128 ± 57 | 136 ± 66 | 136 ± 72 |
| DC-LAMP | 58 ± 2 | 54 ± 5 | 78 ± 10 | 88 ± 2 |
Results are mean fluorescence intensity (MFI) plus or minus the SEM from 10 (HLA-DR) to 3 (DC-LAMP) experiments.
iDCs were generated from peripheral monocytes by incubation for 6 days with IL-4 and granulocyte-macrophage colony-stimulating factor as reported in “Materials and methods.” On day 6, iDCs were harvested and incubated for 24 hours in the presence (iDCZol+) or absence of 5 μM Zol (iDCZol−).
mDCs were generated by incubation of iDCs with TNF-α and IL-1β for 24 hours in the presence (mDCZol+) or absence (mDCZol−) of 5 μM Zol. DC-LAMP expression is determined after 48 hours of incubation.
Difference statistically significant between mDCZol− and mDCZol+ (both P < .05).