Table 1 Derivation of endothelial cells... | American Society of Hematology

Table 1

Derivation of endothelial cells from hESCs

Reference	ES source	EC derivation
Kaufman²⁴	hES cells (H1, H1.1, and H9.2)	Undifferentiated hESCs were grown on mouse bone marrow cells (S17) or mouse yolk sac endothelial cells (c166) for 17 days.
Wang³³	Human EBs (H9, H1) days 9-10	EBs were sorted by FACS for VE-cadherin/PECAM1⁺ cells and then the sorted cells were grown for 7 days in endothelial-supporting media containing pituitary extracts and VEGF.
Levenberg et al²⁹	Human EBs (H9) day 13	FACS sorting for PECAM1⁺ cells.
Gerecht-Nir et al³⁶	hES cells (H9, H13, I9)	Undifferentiated hESCs were grown on IV collagen–coated dishes, filtered (40 μm mesh), and then recultured on IV collagen–coated dishes supplanted with VEGF.
Vodyanik⁴¹	Undifferentiated hES cells (H1, H9)	Undifferentiated hES cells were grown on mouse bone marrow stromal cells (OP9, S17, and MS-5) for 10 days.
Zambidis³⁴	Human EBs (H1) days 7-9	EBs were grown on methylcellulose and supplemented with 50 μg/mL ascorbic acid, 0.5% insulin/transferring/selenium.

Reference	ES source	EC derivation
Kaufman²⁴	hES cells (H1, H1.1, and H9.2)	Undifferentiated hESCs were grown on mouse bone marrow cells (S17) or mouse yolk sac endothelial cells (c166) for 17 days.
Wang³³	Human EBs (H9, H1) days 9-10	EBs were sorted by FACS for VE-cadherin/PECAM1⁺ cells and then the sorted cells were grown for 7 days in endothelial-supporting media containing pituitary extracts and VEGF.
Levenberg et al²⁹	Human EBs (H9) day 13	FACS sorting for PECAM1⁺ cells.
Gerecht-Nir et al³⁶	hES cells (H9, H13, I9)	Undifferentiated hESCs were grown on IV collagen–coated dishes, filtered (40 μm mesh), and then recultured on IV collagen–coated dishes supplanted with VEGF.
Vodyanik⁴¹	Undifferentiated hES cells (H1, H9)	Undifferentiated hES cells were grown on mouse bone marrow stromal cells (OP9, S17, and MS-5) for 10 days.
Zambidis³⁴	Human EBs (H1) days 7-9	EBs were grown on methylcellulose and supplemented with 50 μg/mL ascorbic acid, 0.5% insulin/transferring/selenium.

Summary of experiments in which hESCs were used to derive endothelial potential, indicating the source of the hESCs and the derivation procedure.