Inhibition of Plg binding to murine and human macrophage-related cells by Plg-R Fab
| Plg-R . | Inhibition of Plg binding, % . | ||||
|---|---|---|---|---|---|
| RAW 264.7 . | J774A.1 . | Mouse mФs . | THP-1 . | PMA+THP-1 . | |
| H2B | 50.7 ± 4 | 42 ± 3 | 48 ± 5 | ND | 58 ± 9 |
| α-Enolase | 23 ± 3.5 | ND | 18 ± 2 | 12 ± 3 | 17 ± 7 |
| Annexin 2 | 23 ± 3 | ND | 20 ± 3 | ND | 25 ± 3 |
| p11 | 16 ± 2 | ND | 24 ± 4 | ND | 29 ± 5 |
| Plg-R . | Inhibition of Plg binding, % . | ||||
|---|---|---|---|---|---|
| RAW 264.7 . | J774A.1 . | Mouse mФs . | THP-1 . | PMA+THP-1 . | |
| H2B | 50.7 ± 4 | 42 ± 3 | 48 ± 5 | ND | 58 ± 9 |
| α-Enolase | 23 ± 3.5 | ND | 18 ± 2 | 12 ± 3 | 17 ± 7 |
| Annexin 2 | 23 ± 3 | ND | 20 ± 3 | ND | 25 ± 3 |
| p11 | 16 ± 2 | ND | 24 ± 4 | ND | 29 ± 5 |
RAW 264.7 cells, J774A.1 cells, TG-induced peritoneal mouse macrophages (mФs), THP-1 (human monocytoid), or PMA-stimulated THP-1 (macrophage-like) cells were preincubated with Fab to H2B (C-terminal), α-enolase, annexin 2, or anti-p11 for 30 minutes at 4°C, followed by incubation with Alexa 488–labeled Plg (200 nM) for 60 minutes. The cells were washed and bound Plg was measured by FACS. Calculations are based on inhibition of specific Plg binding, inhibitable by EACA. The data are means plus or minus SD from 3 independent experiments.
ND indicates no detectable inhibition.