B-cell population sizes
. | Population size . | . | |
|---|---|---|---|
. | Control . | β1β7 mutant . | |
| BM | |||
| B220med | 5.2 ± 1.6 | 5.5 ± 1.2 | |
| B220hi | 5.7 ± 1.8 | 4.7 ± 1.1 | |
| CD19 | 12.9 ± 6.2 | 10.5 ± 2.5 | |
| IgM | 6.0 ± 1.1 | 5.1 ± 1.4 | |
| IgD | 8.5 ± 6.5 | 4.7 ± 1.1 | |
| Spleen | |||
| B220hi | 40.5 ± 12.0 | 37.7 ± 12.8 | |
| CD19 | 38.9 ± 13.9 | 36.9 ± 10.5 | |
| IgM | 34.6 ± 3.5 | 32.8 ± 9.3 | |
| IgD | 39.7 ± 1.6 | 45.5 ± 15.7 | |
| LN | |||
| B220hi | 19.8 ± 4.0 | 18.3 ± 4.5 | |
| CD19 | 17.4 ± 4.3 | 16.1 ± 5.5 | |
| IgM | 8.4 ± 3.7 | 9.0 ± 3.5 | |
| IgD | 15.0 ± 1.7 | 16.7 ± 7.0 | |
. | Population size . | . | |
|---|---|---|---|
. | Control . | β1β7 mutant . | |
| BM | |||
| B220med | 5.2 ± 1.6 | 5.5 ± 1.2 | |
| B220hi | 5.7 ± 1.8 | 4.7 ± 1.1 | |
| CD19 | 12.9 ± 6.2 | 10.5 ± 2.5 | |
| IgM | 6.0 ± 1.1 | 5.1 ± 1.4 | |
| IgD | 8.5 ± 6.5 | 4.7 ± 1.1 | |
| Spleen | |||
| B220hi | 40.5 ± 12.0 | 37.7 ± 12.8 | |
| CD19 | 38.9 ± 13.9 | 36.9 ± 10.5 | |
| IgM | 34.6 ± 3.5 | 32.8 ± 9.3 | |
| IgD | 39.7 ± 1.6 | 45.5 ± 15.7 | |
| LN | |||
| B220hi | 19.8 ± 4.0 | 18.3 ± 4.5 | |
| CD19 | 17.4 ± 4.3 | 16.1 ± 5.5 | |
| IgM | 8.4 ± 3.7 | 9.0 ± 3.5 | |
| IgD | 15.0 ± 1.7 | 16.7 ± 7.0 | |
For all populations, n = 4. Single-cell suspensions from BM, spleen, and LN of control and β1β7 mutant BM chimeras 2 months after polylC treatment were prepared; stained with antibodies against B220, CD19, IgM, IgD (med indicates medium; hi, high); and β1 integrin and analyzed by FACS. The averages of the population sizes in the respective tissues are shown with standard deviations.