Number of myeloid progenitors derived from PMF-purified CD34+ in methylcellulose medium
| . | EECs . | Total BFU-Es . | Total CFU-GMs . |
|---|---|---|---|
| PMF1 MPL W515L | 0 | 6 | 82 |
| PMF2 MPL W515L | 0 | 2 | 14 |
| PMF3 MPL W515K | 0 | 55 | 185 |
| Mean PMF MPL W515 | 0 | 21 | 71 |
| PMF4 JAK2 V617F | 9 | 84 | 136 |
| PMF5 JAK2 V617F | 28 | 144 | 198 |
| PMF6 JAK2 V617F | 3 | 21 | 17 |
| PMF7 JAK2 V617F | 17 | 17 | 37 |
| PMF8 JAK2 V617F | 3 | 11 | 10 |
| PMF9 JAK2 V617F | 10 | 40 | 150 |
| Mean PMF JAK2 V617F | 12 | 53 | 91 |
| P | .015 | .141 | .387 |
| . | EECs . | Total BFU-Es . | Total CFU-GMs . |
|---|---|---|---|
| PMF1 MPL W515L | 0 | 6 | 82 |
| PMF2 MPL W515L | 0 | 2 | 14 |
| PMF3 MPL W515K | 0 | 55 | 185 |
| Mean PMF MPL W515 | 0 | 21 | 71 |
| PMF4 JAK2 V617F | 9 | 84 | 136 |
| PMF5 JAK2 V617F | 28 | 144 | 198 |
| PMF6 JAK2 V617F | 3 | 21 | 17 |
| PMF7 JAK2 V617F | 17 | 17 | 37 |
| PMF8 JAK2 V617F | 3 | 11 | 10 |
| PMF9 JAK2 V617F | 10 | 40 | 150 |
| Mean PMF JAK2 V617F | 12 | 53 | 91 |
| P | .015 | .141 | .387 |
Peripheral blood CD34+ cells from PMF patients was seeded in methylcellulose in the presence or absence of Epo or G-CSF. Erythroid and granulocytic colonies were picked on day 14 and genotyped by SNP analysis.
EECs indicates endogenous erythroid colonies; BFU-Es, burst-forming units–erythroid; and CFU-GMs, colony-forming units–granulocyte-macrophage.