Tsg inhibits cytokine production
Culture conditions . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . |
---|---|---|---|---|
Media | 20 (3) | 15 (2) | 1.5 (0.6) | 8 (2) |
Anti-CD3 | 75 (8) | 345 (42) | 5 (1) | 119 (14) |
Anti-CD3 + Tsg | 43 (5) | 175 (12) | 2.8 (0.8) | 32 (3) |
Anti-CD3 + TGF-β | 40 (5) | 150 (18) | 2.6 (0.8) | 21 (2) |
Anti-CD3 + Tsg + TGF-β | 25 (3) | 85 (11) | 1.5 (0.6) | 10 (2) |
Culture conditions . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . |
---|---|---|---|---|
Media | 20 (3) | 15 (2) | 1.5 (0.6) | 8 (2) |
Anti-CD3 | 75 (8) | 345 (42) | 5 (1) | 119 (14) |
Anti-CD3 + Tsg | 43 (5) | 175 (12) | 2.8 (0.8) | 32 (3) |
Anti-CD3 + TGF-β | 40 (5) | 150 (18) | 2.6 (0.8) | 21 (2) |
Anti-CD3 + Tsg + TGF-β | 25 (3) | 85 (11) | 1.5 (0.6) | 10 (2) |
CD4+ T cells were cultured under the indicated culture conditions, and cytokine concentrations were analyzed by ELISA in the supernatants of 24 hours of culture. Results show concentrations in picograms per milliliter and are representative of 3 independent experiments. The concentrations used were as follows: anti-CD3, 100 ng/mL; Tsg, 2 μg; TGF-β, 2 μg/mL. The lowest limits of ELISA detection were as follows: IL-2, 6 pg/mL; IFN-γ, 8 pg/mL; IL-4, 0.13 pg/mL; IL-10, 3.9 pg/mL.
Standard deviations of the values are shown in parentheses.