Summary of HIV-1 infection in the DKO-HSC mice
| Mouse no. . | CD34+ cells . | Time after transplantation, wk . | HIV (ng/mouse)* . | HIV RNA per mL blood (CD4/CD8 ratio)† . | ||||
|---|---|---|---|---|---|---|---|---|
| −1 wk after infection . | 1 wk after infection . | 2 wk after infection . | 3 wk after infection . | 4 wk after infection‡ . | ||||
| 1 | CB | 16 | NL4-R3A (5) | ND (6.8) | 4.0 × 105 (0.6) | 2.4 × 104 (0.3) | 3.7 × 103§ | 8.5 × 103§ |
| 2 | CB | 16 | NL4-R3A (5) | ND (1.0) | 2.0 × 103 (0.5) | 2.6 × 104 (0.0) | 6.9 × 103§ | 4.2 × 103§ |
| 3 | CB | 15 | NL4-R3A (1) | ND (3.0) | 9.7 × 103 (2.9) | 9.9 × 104 (1.7) | 1.3 × 105 (0.2) | 1.9 × 104 (3.7) |
| 4 | FL | 19 | NL4-R3A (1) | ND (1.6) | 3.2 × 103 (0.5) | 2.5 × 103 (0.8) | 2.1 × 103 (0.1) | ND§ |
| 5 | FL | 19 | NL4-R3A (1) | ND (1.7) | 4.0 × 103 (1.1) | — | — | — |
| 6 | FL | 41 | NL4-R3A (1) | ND (13.5) | 1.0 × 105 (1.8) | — | — | — |
| 7 | FL | 22 | JR-CSF (1) | ND (4.0) | 1.8 × 104 (5.1) | 2.1 × 105 (2.5) | — | — |
| 8 | FL | 22 | JR-CSF (1) | ND (3.6) | 2.4 × 105 (3.7) | 4.0 × 105 (3.4) | — | — |
| 9 | CB | 17 | NL4-3 (1) | ND (2.7) | 7.8 × 104 (0.9) | 7.6 × 103 (0.8) | — | 8.2 × 104 (0.3) |
| 10 | CB | 17 | NL4-3 (1) | ND (0.9) | ND (1.5) | ND (3.0) | — | — |
| 11 | — | — | NL4-R3A (1) | ND§ | ND§ | ND§ | ND§ | ND§ |
| 12 | — | — | NL4-R3A (1) | ND§ | ND§ | ND§ | ND§ | ND§ |
| 13 | CB | 16 | Mock | ND (1.3) | ND (2.4) | ND (1.7) | ND (3.2) | ND (3.6) |
| 14 | CB | 16 | Mock | ND (1.1) | ND (2.2) | ND (1.7) | — | ND (2.0) |
| Mouse no. . | CD34+ cells . | Time after transplantation, wk . | HIV (ng/mouse)* . | HIV RNA per mL blood (CD4/CD8 ratio)† . | ||||
|---|---|---|---|---|---|---|---|---|
| −1 wk after infection . | 1 wk after infection . | 2 wk after infection . | 3 wk after infection . | 4 wk after infection‡ . | ||||
| 1 | CB | 16 | NL4-R3A (5) | ND (6.8) | 4.0 × 105 (0.6) | 2.4 × 104 (0.3) | 3.7 × 103§ | 8.5 × 103§ |
| 2 | CB | 16 | NL4-R3A (5) | ND (1.0) | 2.0 × 103 (0.5) | 2.6 × 104 (0.0) | 6.9 × 103§ | 4.2 × 103§ |
| 3 | CB | 15 | NL4-R3A (1) | ND (3.0) | 9.7 × 103 (2.9) | 9.9 × 104 (1.7) | 1.3 × 105 (0.2) | 1.9 × 104 (3.7) |
| 4 | FL | 19 | NL4-R3A (1) | ND (1.6) | 3.2 × 103 (0.5) | 2.5 × 103 (0.8) | 2.1 × 103 (0.1) | ND§ |
| 5 | FL | 19 | NL4-R3A (1) | ND (1.7) | 4.0 × 103 (1.1) | — | — | — |
| 6 | FL | 41 | NL4-R3A (1) | ND (13.5) | 1.0 × 105 (1.8) | — | — | — |
| 7 | FL | 22 | JR-CSF (1) | ND (4.0) | 1.8 × 104 (5.1) | 2.1 × 105 (2.5) | — | — |
| 8 | FL | 22 | JR-CSF (1) | ND (3.6) | 2.4 × 105 (3.7) | 4.0 × 105 (3.4) | — | — |
| 9 | CB | 17 | NL4-3 (1) | ND (2.7) | 7.8 × 104 (0.9) | 7.6 × 103 (0.8) | — | 8.2 × 104 (0.3) |
| 10 | CB | 17 | NL4-3 (1) | ND (0.9) | ND (1.5) | ND (3.0) | — | — |
| 11 | — | — | NL4-R3A (1) | ND§ | ND§ | ND§ | ND§ | ND§ |
| 12 | — | — | NL4-R3A (1) | ND§ | ND§ | ND§ | ND§ | ND§ |
| 13 | CB | 16 | Mock | ND (1.3) | ND (2.4) | ND (1.7) | ND (3.2) | ND (3.6) |
| 14 | CB | 16 | Mock | ND (1.1) | ND (2.2) | ND (1.7) | — | ND (2.0) |
Time after transplantation refers to initiation of HIV-1 infection.
ND indicates nondetectable, below the detection level (< 500 copies/mL); —, not done.
The NL4-R3A and NL4 were titered on Hela-CD4-LTR-β-gal cell line. For NL4-R3A, 1 ng p24 equals 4000 infection units. For NL4-3, 1 ng p24 equals 500 infection units. JRCSF was titered on U373-MAGI-CCR5E cells, 1 ng p24 equals 20 infection units. (Both cell lines obtained from NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID.)
Percentage of CD4+ cells divided by percentage of CD8+ in the blood.
Mouse no. 3 sample is from 5 weeks after infection.
No human CD45+ cells detected in the blood.