Table 1 Sequence of oligonucleotides... | American Society of Hematology

Table 1

Sequence of oligonucleotides

Gene	Forward primer	Reverse primer	Cycle no.	Product size, bp
CD31	atc att tct agc gca tgg cct ggt	att tgt gga ggg cga ggt cat aga	36	159
CD34	aaa tcc tct tcc tct gag gct gga	aag agg cag ctg gtg ata agg gtt	36	216
C-FMS	aca gga cct ctt agt ctc tgc cct at	agt ttg tgc ttc ctg ctt ggt gtg	34	196
FOXA2	cca ttg ctg ttg ttg cag gga agt	cac cgt gtc aag att ggg aat gct	28	196
GATA1	tta gcc acc tca tgc ctt tcc ct	cca gag act tgg gtt gtc cag aat	36	197
KDR	cct cta ctc cag taa acc tga ttg gg	tgt tcc cag cat ttc aca cta tgg	32	219
Neuro D	ccc atg gtg ggt tgt cat ata ttc atg t	cca gca tca cat ctc aaa cag cac	40	200
OCT4	aac ctg gag ttt gtg cca ggg ttt	tga act tca cct tcc ctc caa cca	30	123
P1H12	ggg agc aga caa aga tga ggt cta ca	aca tag aca gac aca cac acc cgt	32	184
RUNX1	atg tgg tcc tat tta agc cag ccc	tca tct ggc tga aga cac cag ctt	34	170
SCL	aag ggc aca gca tct gta gtc a	aag tct tca gca gag ggt cac gta	34	104
SOX7	tga caa ctt gtt gcc aac tcc ctg	ttc agc agt gga gga aga gca gaa	28	216
T	tgt ccc agg tgg ctt aca gat gaa	ggt gtg cca aag ttg cca ata cac	32	144
VE-cadherin	tgg aga agt ggc atc agt caa cag	tct aca atc cct tgc agt gtg ag	30	118
β-Actin	ttt gaa tga tga gcc ttc gtc ccc	ggt ctc aag tca gtg tac agg taa gc	25	129
β-Globin	tgt cca ctc ctg atg ctg tta tgg	agc tta gtg ata ctt gtg ggc cag	26	302
ε-Globin	cac tag cct gtg gag caa gat gaa	aat cac cat cac gtt acc cag gag	26	304
γ-Globin	cgc ttc tgg aac gtc tga ggt tat	cca gga gct tga agt tct cag gat	26	370

Gene	Forward primer	Reverse primer	Cycle no.	Product size, bp
CD31	atc att tct agc gca tgg cct ggt	att tgt gga ggg cga ggt cat aga	36	159
CD34	aaa tcc tct tcc tct gag gct gga	aag agg cag ctg gtg ata agg gtt	36	216
C-FMS	aca gga cct ctt agt ctc tgc cct at	agt ttg tgc ttc ctg ctt ggt gtg	34	196
FOXA2	cca ttg ctg ttg ttg cag gga agt	cac cgt gtc aag att ggg aat gct	28	196
GATA1	tta gcc acc tca tgc ctt tcc ct	cca gag act tgg gtt gtc cag aat	36	197
KDR	cct cta ctc cag taa acc tga ttg gg	tgt tcc cag cat ttc aca cta tgg	32	219
Neuro D	ccc atg gtg ggt tgt cat ata ttc atg t	cca gca tca cat ctc aaa cag cac	40	200
OCT4	aac ctg gag ttt gtg cca ggg ttt	tga act tca cct tcc ctc caa cca	30	123
P1H12	ggg agc aga caa aga tga ggt cta ca	aca tag aca gac aca cac acc cgt	32	184
RUNX1	atg tgg tcc tat tta agc cag ccc	tca tct ggc tga aga cac cag ctt	34	170
SCL	aag ggc aca gca tct gta gtc a	aag tct tca gca gag ggt cac gta	34	104
SOX7	tga caa ctt gtt gcc aac tcc ctg	ttc agc agt gga gga aga gca gaa	28	216
T	tgt ccc agg tgg ctt aca gat gaa	ggt gtg cca aag ttg cca ata cac	32	144
VE-cadherin	tgg aga agt ggc atc agt caa cag	tct aca atc cct tgc agt gtg ag	30	118
β-Actin	ttt gaa tga tga gcc ttc gtc ccc	ggt ctc aag tca gtg tac agg taa gc	25	129
β-Globin	tgt cca ctc ctg atg ctg tta tgg	agc tta gtg ata ctt gtg ggc cag	26	302
ε-Globin	cac tag cct gtg gag caa gat gaa	aat cac cat cac gtt acc cag gag	26	304
γ-Globin	cgc ttc tgg aac gtc tga ggt tat	cca gga gct tga agt tct cag gat	26	370