Hemodynamic and microvascular parameters of cremaster muscle venules in TNF-α–induced inflammation
| TNF-α treatment/mouse genotype . | Mice (n) . | Venules (n) . | Diameter, μm . | Centerline velocity, mm/s . | Wall shear rate, s−1 . |
|---|---|---|---|---|---|
| 2.5 hours | |||||
| WT | 5 | 35 | 36.2 ± 1.2 | 2.0 ± 0.1 | 590 ± 17 |
| L-selectin−/− | 6 | 49 | 36.5 ± 0.9 | 2.0 ± 0.1 | 589 ± 12 |
| PSGL-1−/− | 5 | 29 | 36.0 ± 1.5 | 1.9 ± 0.1 | 567 ± 15 |
| DKO | 4 | 41 | 35.6 ± 1.0 | 2.0 ± 0.1 | 590 ± 17 |
| 6 hours | |||||
| WT | 4 | 28 | 35.2 ± 1.1 | 1.7 ± 0.1 | 520 ± 25 |
| L-selectin−/− | 4 | 22 | 29.9 ± 1.6 | 1.4 ± 0.1 | 516 ± 28 |
| PSGL-1−/− | 3 | 24 | 29.2 ± 1.1 | 1.4 ± 0.1 | 522 ± 26 |
| DKO | 4 | 31 | 30.9 ± 1.4 | 1.5 ± 0.1 | 521 ± 29 |
| TNF-α treatment/mouse genotype . | Mice (n) . | Venules (n) . | Diameter, μm . | Centerline velocity, mm/s . | Wall shear rate, s−1 . |
|---|---|---|---|---|---|
| 2.5 hours | |||||
| WT | 5 | 35 | 36.2 ± 1.2 | 2.0 ± 0.1 | 590 ± 17 |
| L-selectin−/− | 6 | 49 | 36.5 ± 0.9 | 2.0 ± 0.1 | 589 ± 12 |
| PSGL-1−/− | 5 | 29 | 36.0 ± 1.5 | 1.9 ± 0.1 | 567 ± 15 |
| DKO | 4 | 41 | 35.6 ± 1.0 | 2.0 ± 0.1 | 590 ± 17 |
| 6 hours | |||||
| WT | 4 | 28 | 35.2 ± 1.1 | 1.7 ± 0.1 | 520 ± 25 |
| L-selectin−/− | 4 | 22 | 29.9 ± 1.6 | 1.4 ± 0.1 | 516 ± 28 |
| PSGL-1−/− | 3 | 24 | 29.2 ± 1.1 | 1.4 ± 0.1 | 522 ± 26 |
| DKO | 4 | 31 | 30.9 ± 1.4 | 1.5 ± 0.1 | 521 ± 29 |
Diameter, centerline velocity, and wall shear rate are presented as the mean plus or minus SEM of all the venules investigated.