Nucleolar disorganization in skin fibroblasts from DBA patients
| . | No. of cells analyzed . | No. of nucleoli per cell . | Surface of nucleoli per cell, μm2 . | Average fluorescence in nucleoli for ITS1, gray level per pixel . | Average fluorescence in nucleoli for U3, gray level per pixel . |
|---|---|---|---|---|---|
| Control | 568 | 3.3 ± 1.2 | 18.0 ± 4.9 | 558 ± 136 | 818 ± 227 |
| MUTS-A | 182 | 2.7 ± 1.1 | 19.7 ± 7.9 | 627 ± 153 | 977 ± 282 |
| MUTS-B | 168 | 3.0 ± 1.2 | 20.6 ± 6.5 | 641 ± 169 | 783 ± 232 |
| NOTS-A | 91 | 2.6 ± 1.1 | 29.4 ± 8.2 | 638 ± 160 | 711 ± 202 |
| . | No. of cells analyzed . | No. of nucleoli per cell . | Surface of nucleoli per cell, μm2 . | Average fluorescence in nucleoli for ITS1, gray level per pixel . | Average fluorescence in nucleoli for U3, gray level per pixel . |
|---|---|---|---|---|---|
| Control | 568 | 3.3 ± 1.2 | 18.0 ± 4.9 | 558 ± 136 | 818 ± 227 |
| MUTS-A | 182 | 2.7 ± 1.1 | 19.7 ± 7.9 | 627 ± 153 | 977 ± 282 |
| MUTS-B | 168 | 3.0 ± 1.2 | 20.6 ± 6.5 | 641 ± 169 | 783 ± 232 |
| NOTS-A | 91 | 2.6 ± 1.1 | 29.4 ± 8.2 | 638 ± 160 | 711 ± 202 |
Semiquantitative analysis of FISH labeling was performed on randomly taken images (see “Material and methods”). Comparison of any measure in DBA cells relative to the corresponding control with Student t test shows a very significant difference (P < .01). Data shown as mean ± standard deviation.