Similar levels of mixed chimerism in GalT−/− mice regardless of CR2 expression on hematopoietic or nonhematopoietic cells
| Group . | . | No. . | Chimerism,* percentage of donor cells . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Phenotype of CR2 chimera . | CD4 . | B220 . | Monocytes . | ||||||
| CR2 expression on stroma . | CR2 expression on BM . | 4 wk after second BMT . | 8 wk after second BMT† . | 4 wk after second BMT . | 8 wk after second BMT† . | 4 wk after second BMT† . | 8 wk after second BMT† . | ||
| GalT KO → GalT KO | + | + | 8 | 27.6 ± 14.4† | 30.2 ± 12.5 | 62.5 ± 10.1‡ | 61.2 ± 11.5 | 87.5 ± 4.9 | 90.2 ± 5.1 |
| GalT KO → GalT Cr2 DKO | + | − | 5 | 32.7 ± 12.6§ | 34.5 ± 11.5 | 61.1 ± 14.2† | 58.2 ± 11.5 | 82.3 ± 4.6 | 89.2 ± 12.2 |
| GalT Cr2 DKO → GalT KO | − | + | 4 | 16.3 ± 5.2§ | 25.4 ± 12.4 | 39.5 ± 14.6‡ | 55.4 ± 14.2 | 86.0 ± 0.9 | 92.1 ± 3.4 |
| GalT Cr2 DKO → GalT Cr2 DKO | − | − | 8 | 14.7 ± 14.7§ | 24.5 ± 13.5 | 43.9 ± 15.8‡ | 52.1 ± 14.2 | 86.6 ± 22.0 | 91.2 ± 8.5 |
| Group . | . | No. . | Chimerism,* percentage of donor cells . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Phenotype of CR2 chimera . | CD4 . | B220 . | Monocytes . | ||||||
| CR2 expression on stroma . | CR2 expression on BM . | 4 wk after second BMT . | 8 wk after second BMT† . | 4 wk after second BMT . | 8 wk after second BMT† . | 4 wk after second BMT† . | 8 wk after second BMT† . | ||
| GalT KO → GalT KO | + | + | 8 | 27.6 ± 14.4† | 30.2 ± 12.5 | 62.5 ± 10.1‡ | 61.2 ± 11.5 | 87.5 ± 4.9 | 90.2 ± 5.1 |
| GalT KO → GalT Cr2 DKO | + | − | 5 | 32.7 ± 12.6§ | 34.5 ± 11.5 | 61.1 ± 14.2† | 58.2 ± 11.5 | 82.3 ± 4.6 | 89.2 ± 12.2 |
| GalT Cr2 DKO → GalT KO | − | + | 4 | 16.3 ± 5.2§ | 25.4 ± 12.4 | 39.5 ± 14.6‡ | 55.4 ± 14.2 | 86.0 ± 0.9 | 92.1 ± 3.4 |
| GalT Cr2 DKO → GalT Cr2 DKO | − | − | 8 | 14.7 ± 14.7§ | 24.5 ± 13.5 | 43.9 ± 15.8‡ | 52.1 ± 14.2 | 86.6 ± 22.0 | 91.2 ± 8.5 |
Recipient mice were treated with anti-CD4 and anti-CD8 mAbs on day –5, TI and TBI on day 0, and BALB/c BMT on day 0. All groups received 2 × 107 BALB/c cells at their second BMT.
Chimerism was detected by FCM analysis and analyzed as in Table 1. The mean and SD percentage of H-2d–positive BALB/c donor cells is shown for the indicated lineages.
NS compared with other groups at the same time point.
P < .02 comparing GalT KO → GalT KO to GalT Cr2 DKO → GalT KO or GalT Cr2 DKO → GalT Cr2 DKO at the same time point.
P < .05 comparing GalT KO → GalT Cr2 DKO to GalT Cr2 DKO → GalT KO or GalT Cr2 DKO → GalT Cr2 DKO at the same time point.