Table 1. KGF administration enhances... | American Society of Hematology

Table 1.

KGF administration enhances thymic recovery after irradiation, dexamethasone, or cyclophosphamide

	Irradiation, day 28			Dexamethasone, day 7
	PBS, × 10⁶	KGF, × 10⁶	PBS, × 10⁶	KGF, × 10⁶	PBS, × 10⁶	KGF, × 10⁶
Thymus	26.5 ± 4.6	68.2 ± 10.4^*	17.7 ± 2.9	47.5 ± 2.0^*	2.7 ± 0.3	24.8 ± 4.8^*
DP	21.3 ± 4.3	54.4 ± 8.9^*	15.2 ± 2.5	39.5 ± 1.8^*	2.0 ± 0.3	20.4 ± 4.1^*
SP-CD4	2.6 ± 0.4	5.9 ± 0.9^*	0.94 ± 0.2	2.45 ± 0.15^*	0.33 ± 0.03	1.98 ± 0.41^*
SP-CD8	0.8 ± 0.1	2.5 ± 0.4^*	0.57 ± 0.12	1.92 ± 0.2^*	0.13 ± 0.01	0.64 ± 0.11^*
DN1	0.1 ± 0.04	0.26 ± 0.1	0.09 ± 0.02	0.11 ± 0.01	0.01 ± 0.0004	0.03 ± 0.003
DN2	0.07 ± 0.03	0.38 ± 0.07^*	0.04 ± 0.01	0.12 ± 0.02^*	0.01 ± 0.001	0.04 ± 0.004
DN3	0.58 ± 0.12	2.15 ± 0.41^*	0.26 ± 0.05	1.07 ± 0.06^*	0.08 ± 0.02	0.49 ± 0.1^*
DN4	0.69 ± 0.14	1.18 ± 0.3^*	0.4 ± 0.09	2.01 ± 0.09^*	0.09 ± 0.02	0.91 ± 0.22^*
Spleen	41.2 ± 6.6	49.0 ± 7.4	69.4 ± 8.9	65.6 ± 5.8	92 ± 6.3	116 ± 14.7
CD4	5.5 ± 0.5	6.5 ± 0.4	11.7 ± 1.5	10.4 ± 1.4	7.2 ± 0.6	8.8 ± 1.4
CD8	2.3 ± 0.2	2.9 ± 0.2	7.4 ± 1.0	5.7 ± 0.7	4.4 ± 0.6	6.0 ± 0.9

	Irradiation, day 28			Dexamethasone, day 7
	PBS, × 10⁶	KGF, × 10⁶	PBS, × 10⁶	KGF, × 10⁶	PBS, × 10⁶	KGF, × 10⁶
Thymus	26.5 ± 4.6	68.2 ± 10.4^*	17.7 ± 2.9	47.5 ± 2.0^*	2.7 ± 0.3	24.8 ± 4.8^*
DP	21.3 ± 4.3	54.4 ± 8.9^*	15.2 ± 2.5	39.5 ± 1.8^*	2.0 ± 0.3	20.4 ± 4.1^*
SP-CD4	2.6 ± 0.4	5.9 ± 0.9^*	0.94 ± 0.2	2.45 ± 0.15^*	0.33 ± 0.03	1.98 ± 0.41^*
SP-CD8	0.8 ± 0.1	2.5 ± 0.4^*	0.57 ± 0.12	1.92 ± 0.2^*	0.13 ± 0.01	0.64 ± 0.11^*
DN1	0.1 ± 0.04	0.26 ± 0.1	0.09 ± 0.02	0.11 ± 0.01	0.01 ± 0.0004	0.03 ± 0.003
DN2	0.07 ± 0.03	0.38 ± 0.07^*	0.04 ± 0.01	0.12 ± 0.02^*	0.01 ± 0.001	0.04 ± 0.004
DN3	0.58 ± 0.12	2.15 ± 0.41^*	0.26 ± 0.05	1.07 ± 0.06^*	0.08 ± 0.02	0.49 ± 0.1^*
DN4	0.69 ± 0.14	1.18 ± 0.3^*	0.4 ± 0.09	2.01 ± 0.09^*	0.09 ± 0.02	0.91 ± 0.22^*
Spleen	41.2 ± 6.6	49.0 ± 7.4	69.4 ± 8.9	65.6 ± 5.8	92 ± 6.3	116 ± 14.7
CD4	5.5 ± 0.5	6.5 ± 0.4	11.7 ± 1.5	10.4 ± 1.4	7.2 ± 0.6	8.8 ± 1.4
CD8	2.3 ± 0.2	2.9 ± 0.2	7.4 ± 1.0	5.7 ± 0.7	4.4 ± 0.6	6.0 ± 0.9

Three-month-old CBA mice received 5 mg/kg KGF or PBS subcutaneously on 3 consecutive days. Mice were sublethally irradiated (450 cGy) and harvested 28 days after KGF administration (irradiation columns). Mice were then treated with either dexamethasone (5 mg/kg 24 hours after the last KGF administration) or cyclophosphamide (300 mg/kg 72 hours after the last KGF administration) and harvested at day 7 and day 10, respectively. Thymic and splenic cellularities were analyzed and thymocytes were stained with anti-CD4, -CD8, -CD3, -CD44, and -CD25 antibodies. Thymic subpopulations were calculated from thymic cellularity and the percentage of particular subsets analyzed by multicolor flow cytometry (DP, CD4⁺ CD8⁺; DN, CD4⁻ CD8⁻; DN1, CD44⁺ CD25⁻ CD4⁻ CD8⁻ CD3⁻; DN2, CD44⁺ CD25⁺ CD4⁻ CD8⁻ CD3⁻; DN3, CD44⁻ CD25⁺ CD4⁻ CD8⁻ CD3⁻; and, DN4, CD44⁻ CD25⁻ CD4⁻ CD8⁻ CD3⁻). Splenocytes were stained with anti-CD3, -CD4, and -CD8 antibodies, and the number of T cells was calculated by splenic cellularity and multicolor flow cytometry. Values represent mean (± SEM) and n = 4-8 mice per group.

Significantly different from control group.

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