Identification of multiple pentadecapeptides eliciting HSV-TK-specific CD8+ CTL responses after HyTK-positive DLI
. | % IFN+ CD8+ T cells . | . | |
|---|---|---|---|
| Patient (HLA type) and immunogenic 15-mer sequence*(no. of peptide) . | % in T-cell line† . | % in PBMCs‡ . | |
| UPN 1574 (A3, A24, B7, B35) | |||
| RRTALRPRRQQEATE25-39 (11) | 3.4 | 0.15 (1.2) | |
| VPEPMTYWRVLGASE81-95 (25) | 3.1 | 0.17 (1.3) | |
| QITMGMPYAVTDAVL125-139 (36) | 3.8 | 0.17 (0.9) | |
| FDRHPIAALLCYPAA161-175 (45) | 5.9 | 0.30 (2.8) | |
| NAGPRPHIGDTLFTL277-291 (74) | 10.6 | 0.29 (0.4) | |
| YDQSPAGCRDALLQL329-343 (87) | 7.8 | 0.20 (1.2) | |
| UPN 4103 (A1, A24, B8, B27) | |||
| GHSNRRTALRPRRQQ21-35 (10) | 8.8 | 0.54 | |
| LLCYPAARYLMGSMT169-183 (47) | 7.2 | 0.31 | |
| HIDRLAKRQRPGERL213-227 (58) | 1.1 | 0.27 | |
| LAMLAAIRRVYGLLA229-243 (62) | 1.7 | 0.34 | |
| PQGAEPQSNAGPRPH269-283 (72) | 1.2 | 0.48 | |
| UPN 7826 (A1, A3, B44, B57) | |||
| PIAALLCYPAARYLM165-179 (46) | 61.4 | 0.15 | |
. | % IFN+ CD8+ T cells . | . | |
|---|---|---|---|
| Patient (HLA type) and immunogenic 15-mer sequence*(no. of peptide) . | % in T-cell line† . | % in PBMCs‡ . | |
| UPN 1574 (A3, A24, B7, B35) | |||
| RRTALRPRRQQEATE25-39 (11) | 3.4 | 0.15 (1.2) | |
| VPEPMTYWRVLGASE81-95 (25) | 3.1 | 0.17 (1.3) | |
| QITMGMPYAVTDAVL125-139 (36) | 3.8 | 0.17 (0.9) | |
| FDRHPIAALLCYPAA161-175 (45) | 5.9 | 0.30 (2.8) | |
| NAGPRPHIGDTLFTL277-291 (74) | 10.6 | 0.29 (0.4) | |
| YDQSPAGCRDALLQL329-343 (87) | 7.8 | 0.20 (1.2) | |
| UPN 4103 (A1, A24, B8, B27) | |||
| GHSNRRTALRPRRQQ21-35 (10) | 8.8 | 0.54 | |
| LLCYPAARYLMGSMT169-183 (47) | 7.2 | 0.31 | |
| HIDRLAKRQRPGERL213-227 (58) | 1.1 | 0.27 | |
| LAMLAAIRRVYGLLA229-243 (62) | 1.7 | 0.34 | |
| PQGAEPQSNAGPRPH269-283 (72) | 1.2 | 0.48 | |
| UPN 7826 (A1, A3, B44, B57) | |||
| PIAALLCYPAARYLM165-179 (46) | 61.4 | 0.15 | |
Position within the sequence of the 376 aa HSV-TK protein (GenBank accession nos. V000467, CAA23741).
T-cell lines were generated by stimulating samples of postinfusion PBMCs obtained from each of the 3 patients with γ-irradiated donor T cells expressing HyTK (UPN 1574, UPN 4103) or HSV-TK (UPN 7826).
IFN-γ+ CD8+ T cells in PBMCs (days 14 to 28) after the first HyTK-positive T-cell infusion. For UPN 1574, the frequency of IFN-γ-producing T cells to each epitope 28 days after infusion 2 is shown in parentheses.