Hematopoietic reconstitution by CXCR4–/– fetal liver cells
Time after transplantation, no. of cells injected/mouse . | % of circulating CD45.2+ cells (no.) . | . | |
|---|---|---|---|
| . | CXCR4+/+ chimeras . | CXCR4-/- chimeras . | |
| 5 weeks | |||
| 2 × 106 | 84.1 ± 5 (8) | 21.6 ± 9 (7) | |
| 5 × 106 | 85 ± 4 (6) | 82 ± 7 (5) | |
| 16 weeks | |||
| 2 × 106 | 96 ± 2 (8) | 15 ± 14 (6) | |
| 5 × 106 | 90.6 ± 1 (6) | 75.3 ± 3 (5) | |
Time after transplantation, no. of cells injected/mouse . | % of circulating CD45.2+ cells (no.) . | . | |
|---|---|---|---|
| . | CXCR4+/+ chimeras . | CXCR4-/- chimeras . | |
| 5 weeks | |||
| 2 × 106 | 84.1 ± 5 (8) | 21.6 ± 9 (7) | |
| 5 × 106 | 85 ± 4 (6) | 82 ± 7 (5) | |
| 16 weeks | |||
| 2 × 106 | 96 ± 2 (8) | 15 ± 14 (6) | |
| 5 × 106 | 90.6 ± 1 (6) | 75.3 ± 3 (5) | |
Freshly isolated cells (CD45.2+) from E14.5 CXCR4+/+ and CXCR4-/- fetal livers were transplanted to adult lethally irradiated C57BL/6-Ly5.1 mice. Two doses of cells have been injected: 2 × 106 or 5 × 106 cells per mouse. Initially, 20 mice were injected with 2 × 106 and 5 with 5 × 106 CXCR4-/- FL cells. In parallel, 9 mice were injected with 2 × 106 and 6 with 5 × 106 CXCR4+/+ FL cells. The recipients were analyzed from 5 weeks to 16 weeks after transplantation. The values represent the mean percentages of circulating CD45.2+ cells observed in peripheral blood samples of CXCR4-/- chimeras and their respective controls. All values are expressed as the mean ± SD.