Information on expression constructs
mRNA . | Plasmid template . | Enzyme . | ng RNA/injection . |
|---|---|---|---|
| lacZ | pGEM-HE-lacZ | HindIII | Varied |
| EF-1α | pTRI-Xef | N/A | Varied |
| xSTAT5 | pSP64T-xSTAT5 | Xbal | 1.0 |
| xSTAT5YF695 | pSP64T-xSTAT5YF695 | Xbal | 1.0 |
| xSTAT5-VP16 | pSP64T-xSTAT-VP16-myc | Xbal | 0.5-0.7 |
| xSTAT5-EnR | pCS2MT-xSTAT5-EnR | Kpnl | 0.8-1.0 |
| myc-xSTAT5 | pCS2MT-xSTAT5 | Kpnl | 1.0 |
| torso-FGFR1 | pSP64T-myc-torso-FGFR1 | Sacl | 1.0 |
| eFGF | pSP64T-eFGF | EcoRl | 0.0005 |
| XFD | pSP64T-XFD | EcoRl | 1.0 |
mRNA . | Plasmid template . | Enzyme . | ng RNA/injection . |
|---|---|---|---|
| lacZ | pGEM-HE-lacZ | HindIII | Varied |
| EF-1α | pTRI-Xef | N/A | Varied |
| xSTAT5 | pSP64T-xSTAT5 | Xbal | 1.0 |
| xSTAT5YF695 | pSP64T-xSTAT5YF695 | Xbal | 1.0 |
| xSTAT5-VP16 | pSP64T-xSTAT-VP16-myc | Xbal | 0.5-0.7 |
| xSTAT5-EnR | pCS2MT-xSTAT5-EnR | Kpnl | 0.8-1.0 |
| myc-xSTAT5 | pCS2MT-xSTAT5 | Kpnl | 1.0 |
| torso-FGFR1 | pSP64T-myc-torso-FGFR1 | Sacl | 1.0 |
| eFGF | pSP64T-eFGF | EcoRl | 0.0005 |
| XFD | pSP64T-XFD | EcoRl | 1.0 |
N/A indicates not applicable.