Table 3. Changes in cell-cycle... | American Society of Hematology

Table 3.

Changes in cell-cycle distribution of K562 cells induced by MVs generated under different conditions of stimulation

	PHA					PMA
	G₀/G₁	S	G₂/M	G₀/G₁	S	G₂/M	G₀/G₁	S	G₂/M
Control cells	52.7 ± 0.6	23.6 ± 2.1	13.9 ± 0.7	53.1 ± 0.3	24.1 ± 0.7	12.7 ± 1.4	52.5 ± 0.9	23.8 ± 1.4	13.8 ± 0.5
MV, 5 μg/mL	53.6 ± 1.4	24.7 ± 0.9	13.1 ± 0.9	54.06 ± 1.8	24.4 ± 1.1	12.4 ± 1.1	ND	ND	ND
MV, 10 μg/mL	53.8 ± 0.5	24.6 ± 2.7	12.8 ± 0.9	53.9 ± 0.3	23.9 ± 0.3	12.7 ± 1.2	64.9 ± 1.9*	13.71 ± 1.1*	11.6 ± 0.9*
MV + @Smo	ND	ND	ND	ND	ND	ND	63.7 ± 2.8*	16.1 ± 0.7*	12.8 ± 0.9*
MV + @control	ND	ND	ND	ND	ND	ND	62.3 ± 3.3*	17.3 ± 0.6*	19.02 ± 1.1*

	PHA					PMA
	G₀/G₁	S	G₂/M	G₀/G₁	S	G₂/M	G₀/G₁	S	G₂/M
Control cells	52.7 ± 0.6	23.6 ± 2.1	13.9 ± 0.7	53.1 ± 0.3	24.1 ± 0.7	12.7 ± 1.4	52.5 ± 0.9	23.8 ± 1.4	13.8 ± 0.5
MV, 5 μg/mL	53.6 ± 1.4	24.7 ± 0.9	13.1 ± 0.9	54.06 ± 1.8	24.4 ± 1.1	12.4 ± 1.1	ND	ND	ND
MV, 10 μg/mL	53.8 ± 0.5	24.6 ± 2.7	12.8 ± 0.9	53.9 ± 0.3	23.9 ± 0.3	12.7 ± 1.2	64.9 ± 1.9*	13.71 ± 1.1*	11.6 ± 0.9*
MV + @Smo	ND	ND	ND	ND	ND	ND	63.7 ± 2.8*	16.1 ± 0.7*	12.8 ± 0.9*
MV + @control	ND	ND	ND	ND	ND	ND	62.3 ± 3.3*	17.3 ± 0.6*	19.02 ± 1.1*

MVs were obtained from CEM T cells treated with either medium (control cells), PHA (MV^Hh-), PMA (MV^Hh-), or PHA/act D (MV^Hh+) and added at the indicated concentrations to K562-cell cultures. In parallel, K562 cells treated with PHA/act D-generated MV (MV^Hh+) were also incubated with anti-Smo (@Smo, 1:150) or preimmune sera (@control, 1:150).

Data are from 7 experiments.

P < .05, significantly different from control cells.

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