Effects of IL-3 and BCR/ABL on histamine levels in Ton.B210-X cells
Culture condition, h 1-24 . | Culture condition, h 25-48 . | Cellular histamine levels, ng/mL . |
|---|---|---|
| Control medium | Control medium | 0.22 ± 0.13 |
| IL-3 (WEHI-CM) | IL-3 (WEHI-CM) | 0.30 ± 0.06 |
| IL-3 (WEHI-CM) | Control medium | 0.28 ± 0.14 |
| BCR/ABL, control medium without IL-3 | BCR/ABL, control medium without IL-3 | 3.74 ± 2.00* |
| IL-3 + BCR/ABL | IL-3 + BCR/ABL | 4.45 ± 2.01* |
| IL-3 + BCR/ABL | BCR/ABL, control medium without IL-3 | 4.32 ± 1.71* |
Culture condition, h 1-24 . | Culture condition, h 25-48 . | Cellular histamine levels, ng/mL . |
|---|---|---|
| Control medium | Control medium | 0.22 ± 0.13 |
| IL-3 (WEHI-CM) | IL-3 (WEHI-CM) | 0.30 ± 0.06 |
| IL-3 (WEHI-CM) | Control medium | 0.28 ± 0.14 |
| BCR/ABL, control medium without IL-3 | BCR/ABL, control medium without IL-3 | 3.74 ± 2.00* |
| IL-3 + BCR/ABL | IL-3 + BCR/ABL | 4.45 ± 2.01* |
| IL-3 + BCR/ABL | BCR/ABL, control medium without IL-3 | 4.32 ± 1.71* |
Ton.B210-X cells were kept in control medium (RPMI 1640 + 10% FCS), in medium containing WEHI-conditioned medium (WEHI-CM) as source of murine IL-3, and/or in the presence of doxycycline to induce expression of BCR/ABL, as indicated. After incubation, cells were harvested and subjected to measurement of cellular histamine levels by RIA. No significant differences in cell numbers were found after 48 hours when comparing different culture conditions. Results represent the mean ± SD of 4 independent experiments
P < .05 compared with control medium