Surface plasmon resonance analysis of anti-CD32B antibodies
. | CD32B monovalent binding constants . | . | . | ||
|---|---|---|---|---|---|
| Antibody . | Ka, M-1s-1 . | Kd, s-1 . | KD, nM . | ||
| 2B6 | 26.1 × 105 ± 12.4 × 105 | 6.9 × 10-4 ± 0.9 × 10-4 | 0.30 ± 0.09 | ||
| ch2B6 | 31.0 × 105 ± 10.3 × 105 | 10.9 × 10-4 ± 1.7 × 10-4 | 0.38 ± 0.14 | ||
| hu2B6-3.5 | 51.2 × 105 ± 9.6 × 105 | 11.4 × 10-4 ± 1.5 × 10-4 | 0.23 ± 0.03 | ||
. | CD32B monovalent binding constants . | . | . | ||
|---|---|---|---|---|---|
| Antibody . | Ka, M-1s-1 . | Kd, s-1 . | KD, nM . | ||
| 2B6 | 26.1 × 105 ± 12.4 × 105 | 6.9 × 10-4 ± 0.9 × 10-4 | 0.30 ± 0.09 | ||
| ch2B6 | 31.0 × 105 ± 10.3 × 105 | 10.9 × 10-4 ± 1.7 × 10-4 | 0.38 ± 0.14 | ||
| hu2B6-3.5 | 51.2 × 105 ± 9.6 × 105 | 11.4 × 10-4 ± 1.5 × 10-4 | 0.23 ± 0.03 | ||
Values are the mean ± SD of at least 3 independent experiments.