Correlation between IL-3R subunit quantitation on AML blasts by FACS (MFI) and QRT-PCR and AML-CFC killing by DT388IL3
. | FACS-MFI . | . | AML-CFC*kill . | |
|---|---|---|---|---|
. | CD123 . | CD131 . | . | |
| QRT-PCR | ||||
| IL-3Rα | 0.095 | – | 0.59† | |
| IL-3Rβc | – | 0.40 | 0.70† | |
| AML-CFC* kill | 0.23 | 0.74† | – | |
. | FACS-MFI . | . | AML-CFC*kill . | |
|---|---|---|---|---|
. | CD123 . | CD131 . | . | |
| QRT-PCR | ||||
| IL-3Rα | 0.095 | – | 0.59† | |
| IL-3Rβc | – | 0.40 | 0.70† | |
| AML-CFC* kill | 0.23 | 0.74† | – | |
Data expressed as correlation coefficients between the values indicated as determined for each of 12 of the 19 AML samples listed on Table 1 and according to “Patients, materials, and methods.”
–indicates not done
Percentage of AML-CFCs killed after 24 hours of exposure to 50 ng/mL DT388IL3
P < .05, Student t test