Fold change of granzyme B mRNA levels in stimulated basophils
. | Medium . | . | IL-3 . | . | α-IgER . | . | IL-5 . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | ||||
| 0 h | 1 | 1 | NA | NA | NA | NA | NA | NA | ||||
| 1 h | ND | ND | 8 | 5 | ND | ND | ND | ND | ||||
| 2 h | ND | ND | 355 | 56 | ND | ND | ND | ND | ||||
| 6 h | 73 | 5 | 6061 | 987 | 481 | 12 | 611 | 10 | ||||
| 18 h | 33 | 2 | 8023 | 316 | ND | ND | ND | ND | ||||
| 48 h | ND | ND | 79 | 5 | ND | ND | ND | ND | ||||
. | Medium . | . | IL-3 . | . | α-IgER . | . | IL-5 . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | ||||
| 0 h | 1 | 1 | NA | NA | NA | NA | NA | NA | ||||
| 1 h | ND | ND | 8 | 5 | ND | ND | ND | ND | ||||
| 2 h | ND | ND | 355 | 56 | ND | ND | ND | ND | ||||
| 6 h | 73 | 5 | 6061 | 987 | 481 | 12 | 611 | 10 | ||||
| 18 h | 33 | 2 | 8023 | 316 | ND | ND | ND | ND | ||||
| 48 h | ND | ND | 79 | 5 | ND | ND | ND | ND | ||||
Basophils were cultured without (medium) or with IL-3 (20 ng/mL), anti-IgE receptor antibody (α-IgER, 200 ng/mL), or IL-5 (20 ng/mL) for the time period (in hours) indicated. Shown are mean values of duplicates from 2 separate experiments (Exp 1; Exp 2) with basophils purified from different donors. Values in table represent fold change in mRNA quantity relative to freshly isolated basophils, which was defined as 1.
NA indicates not applicable; ND, not done.