Summary of reporter gene distribution in transgenic mouse lines
. | . | Expression of β-galactosidase activity . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Transgenic lines exhibiting germline transmission . | Embryo . | . | . | . | . | Adult . | . | . | . | ||||||||
| Transcriptional regulator . | . | Large-vessel EC . | Small-vessel EC . | Bone Marrow . | No expression . | Ectopic . | Large-vessel EC . | Small-vessel EC . | No expression . | Ectopic . | ||||||||
| PPHS | 13 | 0/13 | 0/13 | 0/13 | 6/13 | 7/13 | 0/13 | 0/13 | 8/13 | 5/13 | ||||||||
| -5.5HS/PPHS | 10 | 3/6 | 2/3* | 0/6 | 3/6 | 0/6 | 4/10 | 0/10 | 6/10 | 0/10 | ||||||||
| -5.5HS/SV40P | NA | 4/4 | 1/4 | 2/4 | 0/0 | 0/4 | NA | NA | NA | NA | ||||||||
. | . | Expression of β-galactosidase activity . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Transgenic lines exhibiting germline transmission . | Embryo . | . | . | . | . | Adult . | . | . | . | ||||||||
| Transcriptional regulator . | . | Large-vessel EC . | Small-vessel EC . | Bone Marrow . | No expression . | Ectopic . | Large-vessel EC . | Small-vessel EC . | No expression . | Ectopic . | ||||||||
| PPHS | 13 | 0/13 | 0/13 | 0/13 | 6/13 | 7/13 | 0/13 | 0/13 | 8/13 | 5/13 | ||||||||
| -5.5HS/PPHS | 10 | 3/6 | 2/3* | 0/6 | 3/6 | 0/6 | 4/10 | 0/10 | 6/10 | 0/10 | ||||||||
| -5.5HS/SV40P | NA | 4/4 | 1/4 | 2/4 | 0/0 | 0/4 | NA | NA | NA | NA | ||||||||
The number of transgenic mouse lines exhibiting germ line transmission are given (second of paired values). Of these, the number demonstrating reporter gene expression in given locations are stated (first of paired values). For the -5.5HS/SV40P analysis, only transgenic embryos generated following microinjection were analyzed.
NA indicates not applicable.
Of the 3 embryos exhibiting large-vessel EC expression, 2 of these also showed small-vessel EC reporter gene activity.