Efficiency of SDF-1 knockdown in BCCs
BCCs . | SDF-1α, pg/mL . |
|---|---|
| Untransfected | 84 ± 4 |
| pPMSKH1 transfected | 75 ± 3 |
| pPMSKH1-SDF-1/SR | None detected |
| pPMSKH1-SDF-1/KC | None detected |
BCCs . | SDF-1α, pg/mL . |
|---|---|
| Untransfected | 84 ± 4 |
| pPMSKH1 transfected | 75 ± 3 |
| pPMSKH1-SDF-1/SR | None detected |
| pPMSKH1-SDF-1/KC | None detected |
At 80% confluence, culture media were replaced with fresh media. At confluence (48 hours), the culture media were collected and then quantitated for SDF-1α levels by ELISA. The results are presented as the mean ± SD, n = 5.