Microsatellite analysis of colon mucosa
. | . | . | . | . | . | . | . | . | . | . | Laser-microdissected crypts . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . | . | Tetranucleotides . | . | . | . | |||
| HCT/chemo and patient ID . | Age, y/sex . | Disease . | History of chemo* . | Preparative regimen/last chemo . | Reason for colon biopsy . | D of sampling† . | Pathology of biopsy‡ . | Clinical GI-GvHD stage at sampling . | Overall max GvHD§ . | Whole colon section∥ . | THO-1 . | SEE33 . | D14S120 . | Mononucleotides¶ . | |||
| Allo | |||||||||||||||||
| C1 | 61/F | AML | Yes | FBM | Diar | 18 | N | 0 | 0 | S | S | S | S | S | |||
| C2 | 52/M | AML | Yes | BuCy | Diar | 20 | I° | I° | I° | S | S | MSI | S | S | |||
| C3# | 53/M | CML | No | BuCy | Diar | 30 | I° | I° | I° | S | MSI | NE | S | S | |||
| C4 | 19/M | ALL | Yes | TBI/VC | Diar | 42 | I° | II° | III° | S | MSI | MSI | S | S | |||
| C5 | 68/M | AML | No | FBM | Diar | 46 | III° | III° | III° | S | S | MSI | S | S | |||
| C6 | 59/M | MM | Yes | 2Gy/F | Diar | 46 | III° | III° | III° | NE | S | MSI | S | S | |||
| C7 | 65/M | AML | Yes | FBM | Diar | 49 | II° | II° | III° | S | S | S | S | S | |||
| C8 | 45/F | ALL | Yes | BuCy | Diar | 57 | III° | III° | IV° | S | MSI | MSI | S | S | |||
| C9 | 29/M | CML | Yes | BuCy | Diar | 61 | II° | II° | IV° | MSI | NE | MSI | S | S | |||
| C10 | 61/F | AML | Yes | FBM | Diar | 92 | I° | I° | III° | MSI | MSI | NE | NE | S | |||
| C11 | 20/M | ALL | Yes | TBI/VC | Diar | 148 | N | 0 | Ext | S | NE | S | S | S | |||
| C12 | 52/M | CML | No | FBM | Diar | 175 | I° | I° | I° | MSI | S | MSI | NE | S | |||
| C13 | 45/M | AML | Yes | BuCy | Diar | 182 | II° | II° | Ext | S | MSI | MSI | S | S | |||
| C14 | 36/M | NHL | Yes | BuCy | FU | 277 | N | 0 | 0 | MSI | MSI | S | MSI | S | |||
| C15 | 49/F | CML | No | FBM | Diar | 543 | I° | I° | Ext | S | MSI | MSI | NE | S | |||
| C16 | 45/F | MDS | No | BuCy | Diar | 1089 | Col | 0 | Ext | S | S | S | S | S | |||
| Chemo | |||||||||||||||||
| C17 | 52/F | EsCa | Yes | Vino | Diar | 7 | N | NA | NA | S | S | S | NE | S | |||
| C18 | 20/F | HD | No | HD15 | Diar | 10 | Col | NA | NA | S | NE | S | NE | S | |||
| C19 | 72/F | NHL | Yes | Chlor | FU | 240 | N | NA | NA | S | S | S | S | S | |||
| C20 | 66/F | Col Ca | No | 5-FU | FU | 1805 | N | NA | NA | NE | S | S | S | S | |||
| None | |||||||||||||||||
| C21 | 65/F | HepC | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C22 | 73/M | Healthy | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C23 | 48/M | Healthy | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C24 | 69/F | Divert | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C25 | 39/F | Col irr | NA | NA | A pain | NA | N | NA | NA | S | S | S | S | S | |||
| C26 | 57/F | Divert | NA | NA | A pain | NA | N | NA | NA | S | S | S | S | S | |||
| C27 | 83/F | BrCa | No | NA | Bleed | NA | N | NA | NA | S | S | S | NE | S | |||
| C3# | 53/M | CML | No | NA | A pain | NA | N | NA | NA | S | S | NE | S | S | |||
. | . | . | . | . | . | . | . | . | . | . | Laser-microdissected crypts . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | . | . | . | . | . | Tetranucleotides . | . | . | . | |||
| HCT/chemo and patient ID . | Age, y/sex . | Disease . | History of chemo* . | Preparative regimen/last chemo . | Reason for colon biopsy . | D of sampling† . | Pathology of biopsy‡ . | Clinical GI-GvHD stage at sampling . | Overall max GvHD§ . | Whole colon section∥ . | THO-1 . | SEE33 . | D14S120 . | Mononucleotides¶ . | |||
| Allo | |||||||||||||||||
| C1 | 61/F | AML | Yes | FBM | Diar | 18 | N | 0 | 0 | S | S | S | S | S | |||
| C2 | 52/M | AML | Yes | BuCy | Diar | 20 | I° | I° | I° | S | S | MSI | S | S | |||
| C3# | 53/M | CML | No | BuCy | Diar | 30 | I° | I° | I° | S | MSI | NE | S | S | |||
| C4 | 19/M | ALL | Yes | TBI/VC | Diar | 42 | I° | II° | III° | S | MSI | MSI | S | S | |||
| C5 | 68/M | AML | No | FBM | Diar | 46 | III° | III° | III° | S | S | MSI | S | S | |||
| C6 | 59/M | MM | Yes | 2Gy/F | Diar | 46 | III° | III° | III° | NE | S | MSI | S | S | |||
| C7 | 65/M | AML | Yes | FBM | Diar | 49 | II° | II° | III° | S | S | S | S | S | |||
| C8 | 45/F | ALL | Yes | BuCy | Diar | 57 | III° | III° | IV° | S | MSI | MSI | S | S | |||
| C9 | 29/M | CML | Yes | BuCy | Diar | 61 | II° | II° | IV° | MSI | NE | MSI | S | S | |||
| C10 | 61/F | AML | Yes | FBM | Diar | 92 | I° | I° | III° | MSI | MSI | NE | NE | S | |||
| C11 | 20/M | ALL | Yes | TBI/VC | Diar | 148 | N | 0 | Ext | S | NE | S | S | S | |||
| C12 | 52/M | CML | No | FBM | Diar | 175 | I° | I° | I° | MSI | S | MSI | NE | S | |||
| C13 | 45/M | AML | Yes | BuCy | Diar | 182 | II° | II° | Ext | S | MSI | MSI | S | S | |||
| C14 | 36/M | NHL | Yes | BuCy | FU | 277 | N | 0 | 0 | MSI | MSI | S | MSI | S | |||
| C15 | 49/F | CML | No | FBM | Diar | 543 | I° | I° | Ext | S | MSI | MSI | NE | S | |||
| C16 | 45/F | MDS | No | BuCy | Diar | 1089 | Col | 0 | Ext | S | S | S | S | S | |||
| Chemo | |||||||||||||||||
| C17 | 52/F | EsCa | Yes | Vino | Diar | 7 | N | NA | NA | S | S | S | NE | S | |||
| C18 | 20/F | HD | No | HD15 | Diar | 10 | Col | NA | NA | S | NE | S | NE | S | |||
| C19 | 72/F | NHL | Yes | Chlor | FU | 240 | N | NA | NA | S | S | S | S | S | |||
| C20 | 66/F | Col Ca | No | 5-FU | FU | 1805 | N | NA | NA | NE | S | S | S | S | |||
| None | |||||||||||||||||
| C21 | 65/F | HepC | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C22 | 73/M | Healthy | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C23 | 48/M | Healthy | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C24 | 69/F | Divert | NA | NA | TuScr | NA | N | NA | NA | S | S | S | S | S | |||
| C25 | 39/F | Col irr | NA | NA | A pain | NA | N | NA | NA | S | S | S | S | S | |||
| C26 | 57/F | Divert | NA | NA | A pain | NA | N | NA | NA | S | S | S | S | S | |||
| C27 | 83/F | BrCa | No | NA | Bleed | NA | N | NA | NA | S | S | S | NE | S | |||
| C3# | 53/M | CML | No | NA | A pain | NA | N | NA | NA | S | S | NE | S | S | |||
At the time of colon sampling all patients revealed complete donor chimerism in blood (blood chimerism analysis in patients C1 and C2 was done at day 30).
Chemo indicates chemotherapy; HCT, hematopoietic cell transplantation; GI, gastrointestinal; GvHD, graft-versus-host disease; AML, acute myeloid leukemia; Allo, allogeneic HCT; FBM, fludarabine, carmustine, melphalan (reduced intensity conditioning); Diar, diarrhea; S, stable microsatellites; BuCy, busulphan, cyclophosphamide; MSI, microsatellite instability; CML, chronic myeloid leukemia; NE, not evaluable; ALL, acute lymphatic leukemia; TBI/VC, total body irradiation (12 Gy), etoposide phosphate, cyclophosphamide; MM, multiple myeloma; 2Gy/F: low total body irradiation (2 Gy), fludarabine (reduced intensity conditioning); NHL, non-Hodgkin lymphoma; FU, follow-up; MDS, myelodysplastic syndrome; Col, colon; EsCa, esophagus cancer; Vino, vinorelbine; NA, not applicable; HD, Hodgkin disease; HD15, procarbazine, cyclophosphamide, etoposide phosphate, vincristine, bleomycin; Chlor, chlorambucil; Ca, cancer; 5-FU, 5-fluoruracil; HepC, hepatitis C; TuScr, tumor screening; Div, diverticulosis; irr, irritable; A pain, abdominal pain; BrCa, breast cancer.
History of multiple (≥ 3) myelosuppressive chemotherapies before HCT
Day after HCT or last chemotherapy
N indicates normal; Col, infectious colitis; I to IV, pathologic grading of GvHD
Occurrence of graft-versus-host disease (GvHD) any time after HCT, maximal grade; 0 indicates no GvHD; I-IV, grade acute GvHD; Ext, extensive chronic GvHD
Microsatellite PCR using as template DNA extracted from the whole colon section. In these cases, MSI denotes MSI in at least one marker and S no MSI in all markers
ZP3, BAT26, SRY
A gastric biopsy obtained from patient C3 before transplantation showed no MSI. This patient developed squamous cancer of his tongue 3.5 years after allogeneic HCT (see Table 3)