Hematologic parameters in control and experimental mice before (baseline) and 3 weeks after treatment with pI-pC
Genotype . | No. . | Hgb level, g/L . | MCV, fL . | RDW, % . | Reticulocytes, % . | Spleen weight, % . |
|---|---|---|---|---|---|---|
| Before pl-pC | ||||||
| WT | 12 | 165 ± 4 | 55.3 ± 0.4 | 19.7 ± 0.2 | 2.6 ± 0.2 | — |
| Rac1flox/flox | 6 | 164 ± 3 | 51.8 ± 0.7 | 19.6 ± 0.4 | 2.9 ± 0.3 | — |
| Rac2-/- | 6 | 153 ± 4 | 50.4 ± 0.5a | 21.3 ± 0.4b | 3.4 ± 0.2 | — |
| Rac1flox/flox; Rac2-/- | 12 | 154 ± 3 | 48.8 ± 0.7c | 20.4 ± 0.3 | 3.1 ± 0.2 | — |
| After pl-pC | ||||||
| WT | 12 | 160 ± 3 | 56.9 ± 1.0 | 18.6 ± 0.6 | 3.8 ± 0.3d | 0.36 ± 0.03 |
| Rac1-/- | 6 | 155 ± 2e | 54.2 ± 0.5e | 21.2 ± 0.7 | 3.9 ± 0.2e | 0.43 ± 0.07 |
| Rac2-/- | 6 | 142 ± 5f | 49.6 ± 0.9g | 21.5 ± 0.7 | 4.8 ± 0.5h | 0.37 ± 0.03 |
| Rac1-/-; Rac2-/- | 12 | 128 ± 3ij | 43.3 ± 0.6ij | 28.1 ± 1.4ij | 7.2 ± 0.4ij | 0.72 ± 0.15k |
Genotype . | No. . | Hgb level, g/L . | MCV, fL . | RDW, % . | Reticulocytes, % . | Spleen weight, % . |
|---|---|---|---|---|---|---|
| Before pl-pC | ||||||
| WT | 12 | 165 ± 4 | 55.3 ± 0.4 | 19.7 ± 0.2 | 2.6 ± 0.2 | — |
| Rac1flox/flox | 6 | 164 ± 3 | 51.8 ± 0.7 | 19.6 ± 0.4 | 2.9 ± 0.3 | — |
| Rac2-/- | 6 | 153 ± 4 | 50.4 ± 0.5a | 21.3 ± 0.4b | 3.4 ± 0.2 | — |
| Rac1flox/flox; Rac2-/- | 12 | 154 ± 3 | 48.8 ± 0.7c | 20.4 ± 0.3 | 3.1 ± 0.2 | — |
| After pl-pC | ||||||
| WT | 12 | 160 ± 3 | 56.9 ± 1.0 | 18.6 ± 0.6 | 3.8 ± 0.3d | 0.36 ± 0.03 |
| Rac1-/- | 6 | 155 ± 2e | 54.2 ± 0.5e | 21.2 ± 0.7 | 3.9 ± 0.2e | 0.43 ± 0.07 |
| Rac2-/- | 6 | 142 ± 5f | 49.6 ± 0.9g | 21.5 ± 0.7 | 4.8 ± 0.5h | 0.37 ± 0.03 |
| Rac1-/-; Rac2-/- | 12 | 128 ± 3ij | 43.3 ± 0.6ij | 28.1 ± 1.4ij | 7.2 ± 0.4ij | 0.72 ± 0.15k |
All values are mean ± SEM. Population is 12 for the groups of WT and Mx1CreTg/+; Rac1flox/flox; Rac2-/- mice and 6 for the groups of Mx1CreTg/+; Rac1flox/flox and Rac2-/- mice. Corresponding Rac1 protein levels by densitometry after treatment with pl-pC, at the time these blood counts were obtained, were 99.9 ± 1.8% for the WT mice and 42.3 ± 3.5% (range, 25.6%-56.8%) for the Rac1-/-; Rac2-/- mice. Spleen weight was evaluated for 6 mice per group and is expressed as percent of the body weight.
To convert hemoglobin level from grams per liter to grams per deciliter, divide grams per liter by 10.
— indicates not determined. Spleen size within normal limits was observed for all genotypes before pl-pC treatment.
P < .05 versus WT at baseline.
P < .05 versus WT and Rac1-/- at baseline.
P < .05 versus WT and Rac1-/- at baseline.
P < .05 versus WT at baseline.
P < .05 versus Rac1flox/flox at baseline.
P < .05 versus WT after pl-pC treatment.
P < .05 versus WT and Rac1-/- after pl-pC treatment.
P < .05 versus Rac2-/- at baseline.
P < .05 versus WT, Rac1-/-, and Rac2-/- after pl-pC treatment.
P < .001 versus Rac1flox/flox; Rac2-/- at baseline.
P < .05 versus WT after pl-pC treatment.