Comparison of the effect of HS2 deletions on β-globin gene expression
Deletion size . | Transgenic lines . | Embryonic gene, % . | Fetal gene, % . | Adult gene, % . | Reference . |
|---|---|---|---|---|---|
| Human HS2 | |||||
| 1.9-kb full site | 1 | ϵ: 55 | γ and β: 50 | β: 50 | Peterson et al26 |
| γ: 66 | |||||
| 742-bp core | 4 | ϵ: 0-5 | γ: 0-10 | β: 0-38 | Milot et al25 |
| γ: 0-66 | β: 0-40 | ||||
| 375-bp core | 7 | ϵ: 0-10 | γ: 16-47 | β: 1-32 | Bungert et al21 |
| Mouse HS2 | |||||
| 1.1-kb full site | NA | Ey: 88-100 | Bmaj+min: 75-88 | Bmaj+min: 70 | Fiering et al14 |
| Bh1: 84-100 | |||||
| 328-bp core | NA | Ey:∼100 | Bmaj+min: 75-80 | Bmaj+min: 74-87 | This study |
| Bh1: 90-100 |
Deletion size . | Transgenic lines . | Embryonic gene, % . | Fetal gene, % . | Adult gene, % . | Reference . |
|---|---|---|---|---|---|
| Human HS2 | |||||
| 1.9-kb full site | 1 | ϵ: 55 | γ and β: 50 | β: 50 | Peterson et al26 |
| γ: 66 | |||||
| 742-bp core | 4 | ϵ: 0-5 | γ: 0-10 | β: 0-38 | Milot et al25 |
| γ: 0-66 | β: 0-40 | ||||
| 375-bp core | 7 | ϵ: 0-10 | γ: 16-47 | β: 1-32 | Bungert et al21 |
| Mouse HS2 | |||||
| 1.1-kb full site | NA | Ey: 88-100 | Bmaj+min: 75-88 | Bmaj+min: 70 | Fiering et al14 |
| Bh1: 84-100 | |||||
| 328-bp core | NA | Ey:∼100 | Bmaj+min: 75-80 | Bmaj+min: 74-87 | This study |
| Bh1: 90-100 |
ϵ indicates epsilon globin (HBE1); γ, gamma globin (HBG1); β, beta globin (HBB); NA not applicable.