Table 2.

FACS analysis of the BM cells from the mice that received a transplant of AML1-ETO-transduced wild-type BM cells and AML1-ETO-transduced WT1-Tg BM cells




BM cells*
Tranduced gene, BM
n
c-kit+ Lin-, % (median)
c-kit+ Mac1–/low, % (median)
Mac1+ Gr1low, % (median)
Mac1+ Gr1high, % (median)
Empty      
   Wild type   5   0.17-0.90 (0.77)   3.43-5.43 (3.67)   9.9-17.7 (15.6)   23.1-38.2 (30.9)  
   WT1-Tg   6   0.73-3.90 (2.00)  3.23-8.02 (5.20)   11.5-35.2 (28.4)  37.2-49.0 (43.8) 
AML1-ETO      
   Wild type   6   5.85-13.5 (11.5)  22.7-35.4 (28.3)  22.0-39.1 (32.0)  20.4-32.1 (22.3)  
   WT1-Tg
 
8
 
13.7-69.3 (38.7)
 
35.4-76.6 (60.5)
 
0.2-29.0 (13.9)
 
0.2-12.3 (4.29)
 



BM cells*
Tranduced gene, BM
n
c-kit+ Lin-, % (median)
c-kit+ Mac1–/low, % (median)
Mac1+ Gr1low, % (median)
Mac1+ Gr1high, % (median)
Empty      
   Wild type   5   0.17-0.90 (0.77)   3.43-5.43 (3.67)   9.9-17.7 (15.6)   23.1-38.2 (30.9)  
   WT1-Tg   6   0.73-3.90 (2.00)  3.23-8.02 (5.20)   11.5-35.2 (28.4)  37.2-49.0 (43.8) 
AML1-ETO      
   Wild type   6   5.85-13.5 (11.5)  22.7-35.4 (28.3)  22.0-39.1 (32.0)  20.4-32.1 (22.3)  
   WT1-Tg
 
8
 
13.7-69.3 (38.7)
 
35.4-76.6 (60.5)
 
0.2-29.0 (13.9)
 
0.2-12.3 (4.29)
 
*

GFP-positive gated-in population

P < .05 compared with the mice that received a transplant of empty vector-transduced wild-type BM cells

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