Characteristics of the mice that received a transplant of AML1-ETO-transduced wild-type BM cells and AML1-ETO-transduced WT1-Tg BM cells
. | . | . | . | . | . | FACS analysis of peripheral blood, % (median) . | . | Weight, mg (median) . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Transduced gene, BM . | n . | RBCs, × 1012/L (median) . | WBCs, × 109/L (median) . | Plts, × 109/L (median) . | GFP+ cells, % (median) . | Mac1+ Gr1low* . | Mac1+ Gr1high* . | Spleen . | Liver . | Phenotype . | ||
| Empty | ||||||||||||
| Wild type | 7 | 7.0-12.4 (8.8) | 3.3-17.2 (9.4) | 380-980 (560) | 30.5-91.8 (62.8) | 5.3-12.4 (10.2) | 12.5-80.5 (16.8) | 65-107 (82) | 750-1464 (1280) | Normal | ||
| WT1-Tg | 6 | 5.4-10.6 (8.9) | 4.0-12.1 (7.4) | 330-880 (550) | 31.4-73.7 (61.4) | 8.4-44.3 (18.1)† | 11.2-65.7 (41.2)† | 57-133 (120) | 1070-1556 (1395) | Granulocytosis | ||
| AML1-ETO | ||||||||||||
| Wild type | 8 | 4.5-10.9 (8.9) | 3.4-12.0 (6.5) | 200-900 (510) | 24.4-85.6 (50.8) | 15.4-58.2 (30.3)† | 8.1-51.0 (21.6) | 70-280 (168)† | 722-1900 (1520) | Dysplastic myelopoiesis | ||
| WT1-Tg | 10 | 2.8-9.1 (3.4)† | 3.1-35.1 (1.95)† | 120-370 (170)† | 67.4-96.1 (75.0) | 10.2-38.0 (21.4)† | 2.1-29.5 (3.5)† | 208-540 (345)† | 1335-2510 (2015)† | AML | ||
. | . | . | . | . | . | FACS analysis of peripheral blood, % (median) . | . | Weight, mg (median) . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Transduced gene, BM . | n . | RBCs, × 1012/L (median) . | WBCs, × 109/L (median) . | Plts, × 109/L (median) . | GFP+ cells, % (median) . | Mac1+ Gr1low* . | Mac1+ Gr1high* . | Spleen . | Liver . | Phenotype . | ||
| Empty | ||||||||||||
| Wild type | 7 | 7.0-12.4 (8.8) | 3.3-17.2 (9.4) | 380-980 (560) | 30.5-91.8 (62.8) | 5.3-12.4 (10.2) | 12.5-80.5 (16.8) | 65-107 (82) | 750-1464 (1280) | Normal | ||
| WT1-Tg | 6 | 5.4-10.6 (8.9) | 4.0-12.1 (7.4) | 330-880 (550) | 31.4-73.7 (61.4) | 8.4-44.3 (18.1)† | 11.2-65.7 (41.2)† | 57-133 (120) | 1070-1556 (1395) | Granulocytosis | ||
| AML1-ETO | ||||||||||||
| Wild type | 8 | 4.5-10.9 (8.9) | 3.4-12.0 (6.5) | 200-900 (510) | 24.4-85.6 (50.8) | 15.4-58.2 (30.3)† | 8.1-51.0 (21.6) | 70-280 (168)† | 722-1900 (1520) | Dysplastic myelopoiesis | ||
| WT1-Tg | 10 | 2.8-9.1 (3.4)† | 3.1-35.1 (1.95)† | 120-370 (170)† | 67.4-96.1 (75.0) | 10.2-38.0 (21.4)† | 2.1-29.5 (3.5)† | 208-540 (345)† | 1335-2510 (2015)† | AML | ||
RBCs indicates red blood cells; WBCs, white blood cells; and Plts, platelets.
Mac-1+ Gr-1high populations represent mature segmented-form neutrophils, and Mac-1+ Gr-1low populations represent band-formed neutrophils.
Mac-1+ Gr-1low populations represent metamyelocytes and band-formed neutrophils.
GFP-positive gated-in population
P < .05 compared with the mice that received a transplant of empty vector-transduced wild-type BM cells