CD150+CD48-Sca-1+Lineage-c-kit+ cells from old, reconstituted, and cyclophosphamide/G-CSF-mobilized mice are highly enriched for long-term self-renewing, multipotent HSCs
Donor/experiment . | No. mice that engrafted/no. mice total . | No. mice with long-term multilineage reconstitution/no. mice total . | Frequency of cells that long-term multilineage reconstituted . |
|---|---|---|---|
| Young | |||
| 1 | 8/8 | 7/8 | 1 in 2.0 |
| Old | |||
| 1 | 4/7 | 3/7 | 1 in 5.9 |
| 2 | 6/12 | 5/12 | 1 in 6.1 |
| 3 | 5/9 | 3/9 | 1 in 7.9 |
| 4 | 4/9 | 3/9 | 1 in 7.9 |
| Reconstituted | |||
| 1 | 3/6 | 3/6 | 1 in 4.8 |
| 2 | 12/13 | 10/13 | 1 in 2.6 |
| 3 | 7/9 | 3/9 | 1 in 7.9 |
| Mobilized | |||
| 1 | 5/8 | 5/8 | 1 in 3.6 |
Donor/experiment . | No. mice that engrafted/no. mice total . | No. mice with long-term multilineage reconstitution/no. mice total . | Frequency of cells that long-term multilineage reconstituted . |
|---|---|---|---|
| Young | |||
| 1 | 8/8 | 7/8 | 1 in 2.0 |
| Old | |||
| 1 | 4/7 | 3/7 | 1 in 5.9 |
| 2 | 6/12 | 5/12 | 1 in 6.1 |
| 3 | 5/9 | 3/9 | 1 in 7.9 |
| 4 | 4/9 | 3/9 | 1 in 7.9 |
| Reconstituted | |||
| 1 | 3/6 | 3/6 | 1 in 4.8 |
| 2 | 12/13 | 10/13 | 1 in 2.6 |
| 3 | 7/9 | 3/9 | 1 in 7.9 |
| Mobilized | |||
| 1 | 5/8 | 5/8 | 1 in 3.6 |
Three (CD45.1+) CD50+CD48–Sca-1+lin–c-kit+ cells from old, reconstituted, or day-7 cyclophosphamide/G-CSF–mobilized splenocytes were transplanted intravenously into lethally irradiated recipients (CD45.2+) along with 300 000 CD150– recipient-type cells or 200 000 whole bone marrow cells for radioprotection. There was no apparent difference in the frequency of donor cells that long-term multilineage reconstituted based on the nature of cells used for radioprotection. Recipients were considered long-term multilineage reconstituted if donor-type myeloid, B, and T cells were present for at least 16 weeks after transplantation.