Oligonucleotide sequences used for PCR
. | Oligonucleotides, 5′-3′ . |
|---|---|
| RT-PCR | |
| Human IL-12p35 | |
| Forward | GCCACAGGTCTGCATCCA |
| Reverse | GACCTGGCGGGCTGAGTA |
| Probe | 6FAM-TGGACACATGCTGAGCCGGC-TAMRA |
| Human IL-12p40 | |
| Forward | AGCCTCCTCCTTGTGGCTA |
| Reverse | TGTGCTGGTTTTATCTTTTGTG |
| Probe | 6FAM-CCCTGGCACCCAGCACAATG-TAMRA |
| Human IF144 | |
| Forward | TCTGTTTTCCAAGGGCATGT |
| Reverse | GCCATCTTTCCCGTCTCTAA |
| Probe | 6FAM-AGTGCCCACCAAAGCCTGATGC-BHQ1 |
| Human β-actin | |
| Forward | AGCCTCCTCCTTGTGGCTA |
| Reverse | TGTGCTGGTTTTATCTTTTGTG |
| Probe | 6FAM-CCCTGGCACCCAGCACAATG-TAMRA |
| Murine IL-12p35 | |
| Forward | CTTAGCCAGTCCCGAAACCT |
| Reverse | TTGGTCCCGTGTGATGTCT |
| Probe | 6FAM-TCTGGCCGTCTTCACCATGTCA-BHQ1 |
| Murine IL12p40 | |
| Forward | GTTCAACATCAAGAGCAGTAGCA |
| Reverse | CTGCAGACAGAGACGCCATT |
| Probe | 6FAM-CCCTGACTCTCGGGCAGTGACA-TAMRA |
| Murine β-actin | |
| Forward | TCCTGAGCGCAAGTACTCTGT |
| Reverse | CTGATCCACATCTGCTGGAAG |
| Probe | BHQ2-ATCGGTGGCTCCATCCTGGC-Pulsar650 |
| ChIP | |
| Human IL-12p35 promoter | |
| Forward | GCGAACATTTCGCTTTCATT |
| Reverse | ACTTTCCCGGGACTCTGGT |
| Human IL-12p40 promoter | |
| Forward | GCATACAGTTGTTCCATCC |
| Reverse | CTCTACTCCTTTCTGATGGA |
| Human GAPDH promoter | |
| Forward | TACTAGCGGTTTTACGGGCG |
| Reverse | TCGAACAGGAGGAGCAGAGAGCGA |
. | Oligonucleotides, 5′-3′ . |
|---|---|
| RT-PCR | |
| Human IL-12p35 | |
| Forward | GCCACAGGTCTGCATCCA |
| Reverse | GACCTGGCGGGCTGAGTA |
| Probe | 6FAM-TGGACACATGCTGAGCCGGC-TAMRA |
| Human IL-12p40 | |
| Forward | AGCCTCCTCCTTGTGGCTA |
| Reverse | TGTGCTGGTTTTATCTTTTGTG |
| Probe | 6FAM-CCCTGGCACCCAGCACAATG-TAMRA |
| Human IF144 | |
| Forward | TCTGTTTTCCAAGGGCATGT |
| Reverse | GCCATCTTTCCCGTCTCTAA |
| Probe | 6FAM-AGTGCCCACCAAAGCCTGATGC-BHQ1 |
| Human β-actin | |
| Forward | AGCCTCCTCCTTGTGGCTA |
| Reverse | TGTGCTGGTTTTATCTTTTGTG |
| Probe | 6FAM-CCCTGGCACCCAGCACAATG-TAMRA |
| Murine IL-12p35 | |
| Forward | CTTAGCCAGTCCCGAAACCT |
| Reverse | TTGGTCCCGTGTGATGTCT |
| Probe | 6FAM-TCTGGCCGTCTTCACCATGTCA-BHQ1 |
| Murine IL12p40 | |
| Forward | GTTCAACATCAAGAGCAGTAGCA |
| Reverse | CTGCAGACAGAGACGCCATT |
| Probe | 6FAM-CCCTGACTCTCGGGCAGTGACA-TAMRA |
| Murine β-actin | |
| Forward | TCCTGAGCGCAAGTACTCTGT |
| Reverse | CTGATCCACATCTGCTGGAAG |
| Probe | BHQ2-ATCGGTGGCTCCATCCTGGC-Pulsar650 |
| ChIP | |
| Human IL-12p35 promoter | |
| Forward | GCGAACATTTCGCTTTCATT |
| Reverse | ACTTTCCCGGGACTCTGGT |
| Human IL-12p40 promoter | |
| Forward | GCATACAGTTGTTCCATCC |
| Reverse | CTCTACTCCTTTCTGATGGA |
| Human GAPDH promoter | |
| Forward | TACTAGCGGTTTTACGGGCG |
| Reverse | TCGAACAGGAGGAGCAGAGAGCGA |