Table 1. Effect of Btk inhibition on... | American Society of Hematology

Table 1.

Effect of Btk inhibition on thrombin-evoked actin reorganization and Ca²⁺ mobilization

	Control	LFM-A13
F-actin content, % control	70.7 ± 9.8	110.3 ± 4.36^*
Ca²⁺ release, latency, s	1.64 ± 0.09	1.85 ± 0.14
Ca²⁺ entry, latency, s	2.14 ± 0.09	2.60 ± 0.17†

	Control	LFM-A13
F-actin content, % control	70.7 ± 9.8	110.3 ± 4.36^*
Ca²⁺ release, latency, s	1.64 ± 0.09	1.85 ± 0.14
Ca²⁺ entry, latency, s	2.14 ± 0.09	2.60 ± 0.17†

Human platelets were preincubated for 10 minutes with 10 μM LFM-A13 and then were rapidly mixed with 0.1 U/mL thrombin. F-actin content was determined 0.9 second after thrombin stimulation as described in “Materials and methods.” Results shown are presented as percentage of the F-actin content in resting cells and expressed as mean ± SE of 6 independent experiments.

P < .001. Fura-2 fluorescence was recorded at excitation wavelengths of 340 nm (for Ca²⁺ release) and 360 nm (for Ca²⁺ entry). Values are expressed as mean ± SE of at least 6 runs made on cell preparations from 6 donors and 7 to 10 separate experiments

†

P < .05

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